Effect of extracellular signal-regulated kinase on condylar cartilage degeneration of temporomandibular joint osteoarthritis
Journal Title: Journal of Air Force Medical University - Year 2023, Vol 44, Issue 6
Abstract
Objective To explore the effect of extracellular signal-regulated kinase ( ERK) on condylar cartilage degeneration of temporomandibular joint osteoarthritis (TMJOA) in mice. Methods Thirty-six female C57BL / 6J mice were randomly divided into sham-operated group (Sham group) and unilateral anterior crossbite group (UAC group). Six mice from each group were sacrificed at 3, 7 and 11 weeks after modeling and temporomandibular joint ( TMJ) was extracted. Another 18 mice were randomly divided into Sham + PBS group, UAC + PBS group, and UAC + PD98059 group, and were sacrificed 11 weeks later. HE staining and safranin O staining were used to evaluate the degree of condylar cartilage degeneration. RT-PCR was used to detect the mRNA expression levels of Aggrecan, Pcna and Mmp13. Immunohistochemical staining was used to detect the expression of phosphorylated ERK (p-ERK) in condylar cartilage. Results Compared with Sham group at the same time point, there was a significant decrease in the thickness of cartilage, the percentages of safranin O-positive area and the mRNA expression of Aggrecan and Pcna (P < 0. 01), and a significant increase in the percentages of p-ERK-positive chondrocytes and the mRNA expression of Mmp13 in UAC group (P < 0. 01). Compared with UAC + PBS group, there was a significant increase in the thickness of cartilage, the percentages of safranin O-positive area and the mRNA expression of Aggrecan and Pcna (P < 0. 01), and a significant decrease in the mRNA expression of Mmp13 in UAC + PD98059 group (P < 0. 01). Conclusion ERK is involved in the condylar cartilage degeneration of TMJOA, which can be significantly reversed by blocking ERK-related pathways.
Authors and Affiliations
REN Haozhe, MOU Jing, ZHAO Zixuan, KANG Xinyu, ZHANG Mian, MIAO Hui, YU Shibin, HE Feng
Keywords
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