Effect of Fenoldopam on Cytosolic Nitric Oxide Production in Pkd1−⁄− Isolated Femoral Arteries in Response to Fluid Shear Stress Ex Vivo
Journal Title: Journal of Physiology and Pharmacology Advances - Year 2013, Vol 3, Issue 4
Abstract
Adult polycystic kidney disease (PKD) is a genetic disease resulted from mutation in polycystin 1 or 2 proved to be co-localized to endothelial primary cilia. Primary cilium is machanosensory organelle senses shear stress and mediates nitric oxide (NO) signaling. Hypertension is the main cardiovascular abnormality associated with PKD hypothesized to be caused by endothelial dysfunction resulted from defective NO production. To evaluate the effect of fenoldopam on mechanosensory function and NO production of endothelial cilia.Methods: ex vivo fluid shear stress on isolated femoral arteries from wild type and Pkd1−⁄− mice performed. Cytosolic NO measured as percent change in arteries incubated without (control) or with fenoldopam (10 μM; 16 hrs). Fenoldopam restored 80% of NO production and significantly (P<0.0001) elevated cytosolic NO in wild type (187.84±15.977% vs 300.6±15.311%) and Pkd (110.11±6.455% vs 151.17±5.753%) compared to control. Endothelial ciliary mechanosensory function and NO production restored in Pkd1−⁄− by fenoldopam in response to fluid shear stress.
Authors and Affiliations
S. H. Alkhateeb
Keywords
Related Articles
- EP ID EP104312
- DOI -
- Views 86
- Downloads 0
Pre-Implantation Effects of the Root Bark of Adansonia Digitata Linn in Female Wistar rats
To investigate the pre-implantation effects of Adansonia digitata root bark extract, which is used traditionally for antifertility purposes and known to have uterotropic effects. Four groups, comprised of twelve (12) pre...
Biochemical Investigation of Hepatotoxic effects of Antiretroviral Drugs on Wistar Albino Rats
Hepatotoxic effects of antiretroviral drugs (Stavudine, Lamivudine, and Nevirapine) on wistar albino rats were investigated using biochemical parameters; Alanine aminotransferase (ALT), Aspartate aminotransferase (AST),...
Pharmacoepidemiology of Gentamicin Prescription in Dogs by some Practicing Veterinarians in Nigeria
The objective of this study was to evaluate the prescribing trend of gentamicin in dogs by veterinarians in Nigeria. A retrospective and cross-sectional survey was done in six geopolitical zones of Nigeria. About 150 que...
Effect of Extracorporeal Unfractionated Heparin on Hematological and Electrolyte Markers in Hemodialyzed Patients
End-Stage renal disease is the final stage of chronic kidney disease (CKD), which can be managed by hemodialysis (HD), peritoneal dialysis, or renal transplantation. This study was conducted to investigate the changes in...
In vitro Hemostatic Activity Screening of Sap of Jatropha Multifida L. (Euphorbiaceae) used in Traditional Medicine at Cotonou (Benin)
This study has evaluated the hemostatic potential of Jatropha multifida’s sap as traditional medicine in Benin attributed hemostatic properties to this substance. Several hemostatic tests as Blood Coagulation Time, Quick...