Effect of Papain Enzymes on the Density and Amount of Collagen in Scar Tissue in rats
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 12
Abstract
Introduction: Papaya sap containing papain which is a proteolytic enzymes has the ability to increase collagen degradation so that it is expected to be used for the treatment of keloids. Material and Method: This study was an experimental study with Randomized Controled Trial Post Test Only Design with experimental animal. Scar was exposed with papain enzyme and the changes of density and amount of collagen were evaluated. 30 rats were divided randomly into 10 groups. In group P0, excision was performed after 3 months in scarring without prior injection of papain enzymes. In group P1, the papain enzyme was injected at a dose of 5 mg to scar once; in group P2, the papain enzyme was injected at a dose of 5 mg to scar twice with 7-day interval; in group P3, the papain enzyme was injected at a dose of 5 mg to scar 3 times with 7- day interval; in group P4, the papain enzyme was injected at a dose of 7.5 mg to scar once; in group P5, the papain enzyme was injected at a dose of 7.5 mg to scartwice with 7-day interval; in group P6, the papain enzyme was injected at a dose of 7.5 mg to scar 3 times with 7-day interval; in group P7, the papain enzyme was injected at a dose of 10 mg to scar once; in group P8, the papain enzyme was injected at a dose of 10 mg to scar twice with 7-day interval; in group P9, the papain enzyme was injected at a dose of 10 mg to scar 3 times with 7-day interval. Furthermore the result of scar tissue was excision then divided into 2 parts. Histopathologic preparations with Masson's trichrome were prepared. The collagen density wasdetermined by using Olyvia software. The hydroxyproline content was measured by total collagen assay kit using a spectrophotometer Result: The average collagen density was the control group P0 = 231.073; P1 = 216.987; P2 = 203.480; P3 = 200.973; P4 = 203.120; P5 = 201.343; P6 = 200.627; P7 = 199.257; P8 = 193.480; P9 = 181.020. There was a significant differences on the collagen density between the groups P0, P1, P2, P3, P4, P5, P6, P7, P8 and P9 (p<0.05). The average hydroxyproline levels was the control group P0 = 9.752; P1 = 9.073; P2 = 7.472; P3 = 6.184; P4 = 6.596; P5 = 5.894; P6 = 5.709; P7 = 5.821; P8 = 5.480; P9 = 5.176. Conclusion: Density and amount of Collagen was decreased by papain on scar tissue in rats.
Authors and Affiliations
Fauzan . , Herman Josef
Keywords
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- EP ID EP423869
- DOI 10.9790/0853-1712074753.
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