Efficiency of superovulatory response to P-24 protocol in fixed time embryo transfer in Brahman cattle
Journal Title: Revista MVZ Cordoba - Year 2011, Vol 16, Issue 2
Abstract
Objective. To evaluate the efficiency of superovulatory response to P-24 protocol in fixed time embryo transfer in Brahman cattle. Materials and methods. Twelve Brahman cows donors with more than 60 days postpartum were used. Twenty one cows received a superovulatory treatment based in Protocol P-24. Embryos were collected through the conventional method, and the embryos were classified (IETS). Results. An average of 9.1 embryos were collected, 4.4 were considered for direct transfer, 3.7 were considered for freezing and 3.2 were classified as degenerated. According to the stage embryos were classified as: morulae (32.6%), early blastocyst (38%) and blastocyst (18.5%). Effect was presented (p<0.05) in the number of successive superovulations on the production of frozen embryos; however, there was no effect (p>0.05) on the other variables studied. Effect was presented (p<0.05) in the average production of structures recovered, and transferable and frozen embryos among the donors with an output greater than or equal to 5, and less than 5 transferable embryos. By contrast, there was no effect (p>0.05) on the average production of degenerate embryos and unfertilised oocytes among both groups. Nevertheless, the proportion of these ones is greater in the donors with a lower production of transferable embryos (50 and 22.4%) vs. donor with the highest production (39.3 and 14%) for degenerated embryos and unfertilised oocytes respectively. Conclusions. The Brahman cattle has an efficient superovulatory response to P-24 protocol for fixed time embryo transfer.
Authors and Affiliations
Roger Salgado O, M. Sc *| Univesidad de Córdoba, Facultad de Medicina Veterinaria y Zootecnia, Departamento de Ciencias Pecuarias A.A 354 Montería, Colombia.Correspondencia: rdsalgado@sinú.unicordoba.edu.co., Andrés Mejía A, MVZ| MVZ Ejercicio particular., Pablo Suárez S, MVZ| MVZ Ejercicio particular.
Genetic characterization of the domestic pig (Sus scrofa domestica) in Cerete-Colombia, using microsatellite markers
Objective. The purpose of this study was to characterize a population of domestic pig (Sus scrofa domestica) in Cereté, Córdoba, using 20 microsatellite; calculate heterozygosity per locus and average heterozygosity. Mat...
Effect of sucrose in the production of cellulose by Gluconacetobacter xylinus in static culture
Objective. Determine the effect of sucrose on the productivity of BC by Gluconacetobacter xylinus IFO 13693 in static condition. Materials and methods. The synthesis of bacterial cellulose (BC) by Gluconacetobacter xylin...
Neospora caninum serostatus in dairy cattle of the Northern plains of Antioquia, Colombia
Objectives. To determine Neospora caninum seroprevalence in nonvaccinated dairy cattle from the Northern plains of Antioquia. Materials and methods. A cross-sectional study was conducted to determine the prevalence of ne...
Comparison of direct versus Friedewald methods for determing LDL cholesterol levels in the horse
Objective. To compare the Direct Method vs the Precipitation Friedewald Method for determing LDL cholesterol levels in the horse, specie with HDL pattern. Materials and methods. Blood Samples of 200 overnight fasted hors...
Effect of dietary protein level on the development of juveniles of Macrobrachiun tenellum (Smith, 1871)
Objective. To evaluate the effect of five levels of crude protein (CP) in balanced feed on the survival, growth and feed conversion ratio (FCA) in juveniles of Macrobrachium tenellum. Materials and methods. Juveniles of...