Enhancement of cytokine (IL-2, INF-γ) production in Th1 cells by crude protein extract of Equisetum arvense LINNE
Journal Title: Journal of Analytical Bio-Science - Year 2011, Vol 34, Issue 5
Abstract
We previously reported that the highest cell proliferation-inhibitory effect of a crude protein extract solution of Equisetum arvense LINNE (crude E. arvense protein) on cultured cells was noted in the group treated with the highest concentration of crude E. arvense protein. We also performed in vivo experiments on the life-prolonging effect on mice using L-1210 and B16F1 cells, and observed the highest effect at the lowest crude E. arvense protein concentration in experiments using either cell type. The Equisetaceae family is known to have various physiological effects. In this study, the influence of crude E. arvense protein on immune responses was investigated by measuring interleukin-2 (IL-2) and interferon-γ (IFN-γ) produced by Th1 cells playing the central role in cellular immunity. After 24-hour culture with 0.2 mg/ml of crude E. arvense protein in the presence of 5μg/ml ConA, 1,434.5 pg/ml of IL-2 was produced, showing 1.7 times greater production than that in the control. In cells cultured for 48 hours, 2,130.9 pg/ml was produced by cells treated with 0.2 mg/ml of crude E. arvense protein in the presence of 10μg/ml ConA, showing 1.9 times greater production than that in the control. Regarding the IFN-γ production-enhancing effect, 929.3 pg/ml was produced by cells cultured for 24 hours with 0.2 mg/ml of crude E. arvense protein in the presence of 5μg/ml ConA, suggesting that Th1 cells were activated.
Authors and Affiliations
Jun Yukitake
Keywords
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