Epidermal Growth Factor Receptor Mutations detection by Mutant Specific Immunohistochemistry in North Indian Lung Cancer population
Journal Title: International Journal of Research and Development in Pharmacy & Life Sciences (IJRDPL) - Year 2017, Vol 6, Issue 6
Abstract
Background: Lung cancer is one of the leading causes of cancer-related deaths due to limited treatment options available for advanced-stage disease. The epidermal growth factor receptor is a trans-membrane glycoprotein having an extracellular epidermal growth factor binding domain and an intracellular tyrosine kinase domain which regulates signaling pathways to control cellular proliferation. Material and Methods: Detection of EGFR mutation status of 80 subjects of non-small cell lung cancer of adenocarcinoma subtype had been done by IHC using EGFR mutation specific antibodies. Results: 20 cases were positive for Del E746-A750 mutation, 6 for L858R mutation. IHC analysis shows that 30 cases scored 0, 12 cases scored 2+ and 8 cases scored 3+ in Del E746-A750 mutation while 24 cases scored 0, 1 case scored 2+ and 5 cases scored 3+ in L858R mutation. The mean age of mutation positive is 56±9.50 and negative is 53.37±11.83. 65.38% male and 34.61% female were positive for EGFR mutation. The study shows that 8 (30.77%) ex/ current smokers and 18 (69.2%) nonsmokers were positive for EGFR mutations. Conclusion: Patients who were positive for EGFR mutation can take gefitinib treatment instead of taking other chemotherapy regimen. This is a rapid method for diagnosis of lung cancer patients and helpful in the treatment decision which improve the burden of the disease.
Authors and Affiliations
Priyanka Gaur| Department of Physiology King George’s Medical University, Lucknow, India, Sandeep Bhattacharya| Department of Physiology King George’s Medical University, Lucknow, India, Surya Kant| Department of Respiratory Medicine King George’s Medical University, Lucknow, India, RAS Kushwaha| Department of Respiratory Medicine King George’s Medical University, Lucknow, India, Jyotsana Agarwal| Department of Microbiology, King George’s Medical University, Lucknow, India, Anumesh Kumar Pathak | Department of Pathology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow, India
Keywords
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- EP ID EP16043
- DOI http://dx.doi.org/10.21276/IJRDPL.2278-0238.2017.6(6).2796-2
- Views 313
- Downloads 18
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