Escherichia coli and Salmonella spp. Early Diagnosis and Seasonal Monitoring in the Sewage Treatment Process by EMA-qPCR Method
Journal Title: Polish Journal of Microbiology - Year 2015, Vol 64, Issue 2
Abstract
Numerous studies have recently shown that molecular biology tools can allow for early diagnosis of pathogens and can substitute existing cost and time-taking traditional methods. One of them, the qPCR, is successfully used in microbiology and its utility has been assessed for many different biological materials. The aim of this study was to: 1) determine, optimize and apply qPCR as a method to detect [i]Escherichia coli[/i] and [i]Salmonella[/i] spp. in primary influents and final effluents from municipal wastewater treatment plant 2) define if addition of ethidium bromide monoazide (EMA) before DNA extraction can allow to distinguish between alive and dead bacteria, 3) quantify [i]E. coli[/i] and [i]Salmonella[/i] spp. in wastewater during four seasons by qPCR and traditional spread plate method and determine the correlation between the indicator and pathogenic microorganisms. The obtained results has shown that qPCR can be used as a quantitative method in the diagnosis of investigated bacteria in wastewater with EMA pretreatment as a crucial step for a proper quantitative analysis of the presence of these bacteria in wastewater. Both [i]E. coli[/i] and [i]Salmonella [/i]spp. bacteria species were present in all samples of primary influents and final effluents. Our study shown that the quantity of investigated bacteria is strictly correlated with the season that they were obtained in.
Authors and Affiliations
Małgorzata Kacprzak, Krzysztof Fijałkowski, Anna Grobelak, Karolina Rosikoń, Agnieszka Rorat
Keywords
Related Articles
- EP ID EP81143
- DOI -
- Views 96
- Downloads 0
Characterization of Extended-Spectrum-β-Lactamases Produced by Escherichia coli Strains Isolated from Dogs in Poland
Escherichia coli is a common cause of infections in companion animals. In recent years the increasing prevalence of resistance to β-lactams, including extended-spectrum cephalosporins, antimicrobials frequently used in s...
Participation of TP53 and Bcl-2 proteins in gastric carcinomas associated with [i]Helicobacter pylori[/i] or Epstein-Barr virus (EBV)
In the presented studies p53 and bcl-2 proteins expression were evaluated in samples of gastric carcinomas in patients with [i]Helicobacter pylori[/i] or EBV or without [i]H. pylori[/i]/EBV infection. The studies w...
Expiration Date Prediction of Biocontrol Agent Prepared with Bacillus subtilis B579 Using the Accelerated Aging Method
The expiration date of biopesticidal products is an essential feature of their use and storage. In the present work, the expiration date of biocontrol agent was predicted using the accelerated aging method. The available...
Activity of Natural Polyether Ionophores: Monensin and Salinomycin against Clinical [i]Staphylococcus epidermidis[/i] Strains
[i]Staphylococcus epidermidis[/i], a coagulase-negative staphylococcus, is the most important pathogen responsible for chronic nosocomial infections. These bacteria produce extracellular slime and form biofilms on v...
Effect of Lyophilization on Survivability and Growth Kinetic of Trichoderma Strains Preserved on Various Agriculture By-Products
Growth kinetics of four Trichoderma strains was tested on lignocellulosic by-products in solid state fermentation (SSF). The strains were also analyzed for their survival rate and growth after lyophilization on these car...