Escherichia coli and Salmonella spp. Early Diagnosis and Seasonal Monitoring in the Sewage Treatment Process by EMA-qPCR Method
Journal Title: Polish Journal of Microbiology - Year 2015, Vol 64, Issue 2
Abstract
Numerous studies have recently shown that molecular biology tools can allow for early diagnosis of pathogens and can substitute existing cost and time-taking traditional methods. One of them, the qPCR, is successfully used in microbiology and its utility has been assessed for many different biological materials. The aim of this study was to: 1) determine, optimize and apply qPCR as a method to detect [i]Escherichia coli[/i] and [i]Salmonella[/i] spp. in primary influents and final effluents from municipal wastewater treatment plant 2) define if addition of ethidium bromide monoazide (EMA) before DNA extraction can allow to distinguish between alive and dead bacteria, 3) quantify [i]E. coli[/i] and [i]Salmonella[/i] spp. in wastewater during four seasons by qPCR and traditional spread plate method and determine the correlation between the indicator and pathogenic microorganisms. The obtained results has shown that qPCR can be used as a quantitative method in the diagnosis of investigated bacteria in wastewater with EMA pretreatment as a crucial step for a proper quantitative analysis of the presence of these bacteria in wastewater. Both [i]E. coli[/i] and [i]Salmonella [/i]spp. bacteria species were present in all samples of primary influents and final effluents. Our study shown that the quantity of investigated bacteria is strictly correlated with the season that they were obtained in.
Authors and Affiliations
Małgorzata Kacprzak, Krzysztof Fijałkowski, Anna Grobelak, Karolina Rosikoń, Agnieszka Rorat
Keywords
Related Articles
- EP ID EP81143
- DOI -
- Views 90
- Downloads 0
Epidemiology and Genotyping of Patients with Chronic Hepatitis B: Genotype Shifting Observed in Patients from Central Europe
Background: Knowledge on HBV prevalence and genotype distribution in Europe still is hampered by lack of coherent data sampling, small numbers of patients studied so far, and also modern times migration which influences...
<strong>Prevalence of Urogenital Mycoplasmas Among Men with NGU in Upper Silesia, Poland. Preliminary Study.</strong>
The prevalence of urogenital mycoplasmas in men with NGU in Upper Silesia (Poland) was studied. Mycoplasmas were detected in 36.7% men ([i]Ureaplasma parvum[/i] and [i]Mycoplasma genitalium[/i] were found in 30% and 1...
<strong>Interaction of Gram-Positive and Gram-Negative Bacteria with Ceramic Nanomaterials Obtained by Combustion Synthesis – Adsorption and Cytotoxicity Studies</strong>
This paper presents the interactions of Gram-positive ([i]Staphylococcus aureus[/i]) and Gram-negative ([i]Pseudomonas putida[/i]) bacteria with ceramic materials obtained by combustion synthesis. These studies were cond...
Early KPC-Producing Klebsiella pneumoniae Bacteremia among Intensive Care Unit Patients Non-Colonized upon Admission
Among 140 patients colonized by KPC-producing Klebsiella pneumoniae (KPC-Kp) between fourth and seventh day of Intensive Care Unit stay, 24 developed bacteraemia immediately after colonization. Colistin-resistance of the...
Chemical Composition and Antibacterial Activity of Essential Oils of Two Species of Lamiaceae against Phytopathogenic Bacteria
In this study, we aimed to determine chemical composition and antibacterial activities of [i]Satureja hortensis[/i] and [i]Calamintha nepeta[/i] against to 20 phytopathogenic bacteria causing serious crop loss. The...