Establishment of Loop-mediated Isothermal Amplification Method for Rapid Detection of Haemophilus parasuis
Journal Title: 河南科技大学学报（自然科学版） - Year 2017, Vol 38, Issue 6
Abstract
A loop-mediated isothermal amplification ( LAMP) method was established for the simple, rapid, sensitive and specific detection of Haemophilus parasuis ( Hps) . Toking the 16S rRNA fragment of Hps as the targeting sequence, six specific gene regions were selected, and four specific primes ( c ( F3) 、c ( B3) 、c ( FIP) and c ( BIP) ) were designed in these regions. The LAMP rapid detection method for Hps was established by optimizing the reaction system, and detecting the sensitivity and specificity. The results show that the minimum detectable DNA quantity is 0. 241 pg/μL, when the primers c ( F3) : c ( FIP) or c ( B3) : c ( BIP) is 1 : 4, the concentration of Mg2+ is 2 mmol/L, and the concentration of d NTPs is 6 mmol/L. The sensitivity of the method is 100 times than that of polymerase chain reaction method. Furthermore, the method can only detect Hps, but can not detect Streptococcus, Salmonella, Escherichia coli, Bacillus subtilis, Actinobacillus pleuropneumoniae, Staphylococcus aureus.
Authors and Affiliations
Kui HOU, Ke DING, Shouye LIU, Zuhua YU, Zhanqin ZHAO
Keywords
Related Articles
- EP ID EP478132
- DOI -
- Views 86
- Downloads 0
Bifurcation Analysis of Predator-prey Model
For the problem of predator-prey,a predator-prey model with generalized Holling IV functional response was studied. Under proper conditions,the model may have one degenerate equilibrium and one nondegenerate equilibrium....
Application of Adaptive Wavelet Threshold Algorithm to Balise Signal De-noising
In order to improve the de-noising effect of balise signal,through the multi-resolution analysis characteristics of wave let transform,an improved adaptive threshold quantization method was proposed based on the analysis...
Safety Analysis of Abutment Local Underpinning Under Complex Terrain Conditions
For the railway expansion construction project,existing pedestrian bridge was stabilized through unsymmetrical piles framework,which was local underpinning and anti-slide to ensure safety and stability. A three-dimension...
Competitor Recognition Based on Improved SVM Algorithm in E-commerce
To solve the low accuracy of identification of potential competitors in electronic commerce,an improved( support vector machine,SVM) algorithm was presented to optimize the recognition. By combining the Mercer theorem wi...
Performance Test of Aluminum Alloy Floor Formwork
The uniform load tests were carried out on a single aluminum alloy floor formwork and a model room composite aluminum alloy floor formwork system respectively, and the contrastive analysis was carried out.When the three...