Evaluation of anticancer activity of Melaleuka Alternifolia. (i. e. tea tree oil) on Breast cancer cell line (MDA MB)- An in-vitro study
Journal Title: IP International Journal of Medical Microbiology and Tropical Diseases - Year 2018, Vol 4, Issue 3
Abstract
Introduction and Aims According to Globocan 2012 India along with United States and China collectively accounts for almost one third of the global breast cancer burden India is facing challenging situation due to 1154 increases in incidence and 1382 increase in mortality due to breast cancer during 20082012 The current treatment modality has issues of drug resistance and side effects Many anticancer drugs currently used clinically have been isolated from plant species hence investigation of plant species as a source of experimental therapeutic agents in treating cancer is currently gaining a lot of importance One such naturally available plant extract Melaleuka Alternifolia TTO which belongs to the family of essential oils is a very good antibacterial antifungal antiviral antiprotozoal and antiinflammatory agent But currently there is a lot of importance has been given for its anticancer effect Hence our aim is to evaluate anticancer activity of Melaleuka Alternifolia on Breast cancer cell line MDA MB by MTT assay an in vitro methodMaterial and Methods Before the start of the study ethical clearance was obtained from Institutional Review Board The cytotoxicity checked for Breast cancer cell line MDA MB and NIH 3T3 mouse fibroblasts which were used as a control in our current study These cell lines were procured from NCCS Pune India 1 MTT solution preparation stock solution 5 mg in 1 ml of PBS 2 Cell culture The cell lines were maintained in 96 wells micro titer plate containing MEM media supplemented with 10 heat inactivated fetal calf serum FCS containing 5 of mixture of Gentamicin 10ug Penicillin 100 Units ml and Streptomycin 100gml in presence of 5 CO2 at 37C for 4872 hours 3 Cytotoxicity Assay In vitro growth inhibition effect of test compound was assessed by calorimetric or spectrophotometric determination of conversion of MTT into Formazan blue by living cellsResults The results represent the mean of five readings The IC50 value of tea tree oil for breast cancer cell line MDA MB after 48 hrs was 25gml Spearmans rhos Correlation showed P value Conclusion TTO has a promising anticancer property against Breast cancer cell line MDA MB with its IC50 value 25gml Hence this TTO with its greater efficacy related to its anticancer activity can be brought to the level of clinical trials in the coming futureKeywords Cytotoxicity MTT assay MDAMB cell line Tea tree oil
Authors and Affiliations
Sujata Byahatti, Chetana Bogar, Kishore Bhat, Girish Dandagi
Keywords
Related Articles
- EP ID EP485870
- DOI 10.18231/2581-4761.2018.0038
- Views 76
- Downloads 0
A study on determination of Minimum Inhibitory Concentration (MIC) of Vancomycin of MRSA Isolates and their impact in treatment of MRSA Isolates
Introduction: Staphylococcus aureus is one of the most common causes of nosocomial infections. Methicillin-resistant S. aureus (MRSA) is among the top three clinically important pathogens. The glycopeptide vancomycin is...
A study on the prevalence of vancomycin resistant and intermediate staphylococcus aureus isolated from various clinical Specimen in a tertiary care hospital and detection of their MIC values by E-test
The main antimicrobial used to treat MRSA infections which are life threatening is a glycopeptide antibiotic, Vancomycin. Inturn extensive use of vancomycin in various parts of the world lead to the a rise in Staphylococ...
Ventilator associated pneumonia in intensive care units of a tertiary care centre of Gujarat
Purpose: Healthcare associated pneumonia were reported to be the second most common healthcare associated infection in US intensive care units (ICUs). The mortality attributable to VAP was reported to range between 0 and...
Study of Antifungal susceptibility for Fluconazole and Clotrimazole of Candida Species by disc diffusion method in a Tertiary care centre in Karnataka
Background: Vulvo-vaginitis is a common day to day problem in gynecological practice. Candida albicans is the commonest infectious cause. There is limited data regarding the antifungal susceptibility of yeast causing Vul...
Direct reporting cefazolin from VITEKļ 2 for E. coli, K. pneumoniae and P. mirabilis isolated from urine cultures using new CLSI interpretations
Background: In recent years, Clinical and Laboratory Standard Institute (CLSI) has recommended a series breakpoint changes for cefazolin, including testing it as a surrogate agent for oral cephalosporins for treating unc...