EVALUATION OF ANTIMUTAGENIC POTENTIAL OF RIPE AEGLE MARMELOS FRUIT EXTRACT USING MICRONUCLEUS AND CHROMOSOMAL ABERRATION ASSAY IN MOUSE BONE MARROW
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2011, Vol 2, Issue 5
Abstract
The present study was designed to evaluate the antimutagenic potential of ripe Aegle marmelos fruit, using micronucleus and chromosomal aberration assay in mouse bone marrow. The antimutagenic effect of 50% methanolic extract of ripe Aegle marmelos fruit was assessed using cyclophosphamide induced micronuclei (MN) formation and chromosomal aberration (CA) in mouse. The animals were pre-treated with the AME given intraperitonially (i.p.) at three test doses of 600, 900, 1200 mg/kg body weight. In MN test the three doses provided protection when given 24 hrs prior to a single ip administration of 50mg /kg body weight of cyclophosphamide and the efficacy of the test doses were compared with the positive control group. In CA assay the three doses provided protection when given 24 hours prior to a single ip administration ( 50mg/kg body weight) of cyclophophamide followed by a single ip administration of colchicine (25mg/kg body weight), 2 hours before cell harvesting and efficacy of the test doses were compared with that of the positive control group. The percentage protection was increased in a dose dependent manner. These results demonstrate that AME has got antimutagenic potentilal.
Authors and Affiliations
Supriya Das , Rakesh Agarwal , Ashish Sarkar , Manish Gupta
Keywords
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