Evaluation of the cell-free coelomic fluid of the earthworm Eudrilus euginiae to induce apoptosis in SiHa cell line
Journal Title: Journal of Pharmacy Research - Year 2011, Vol 4, Issue 10
Abstract
The cell-free coelomic fluid (CF) was collected from the earthworm Eudrilus euginiae and tested for toxicity at 20, 40, 60, 80 and 100 µl.ml -1 concentrations by MTT assay in SiHa cells invitro. Percentage of inhibition for SiHa cells invitro by CF recorded upto78.52% at 100 µl.ml -1 concentration in 48 hours. AO/EB and Hoechst 33258 staining showed signs of necrosis at 100 µl.ml -1 but morphological changes of typical apoptosis was observed at 20 and 40 µl.ml -1 concentrations. Apoptotic rate increased from 38.58 % to 59.21 %, when repeated at 40 and 50 µl.ml -1 concentrations of CF in 48 hours. Similar increase of apoptotic rate from 68.91% to 79.32% was observed with the increase in the incubation period from 48 h to 72 h suggesting that inducement of apoptosis in SiHa cells invitro by the CF was a function of dose and time period. SDS-PAGE analysis of the CF revealed the presence of a 42 KDa protein – identified earlier to be a tunorilytic factor analogous to the vertebrate cytokine Tumor Necrosis Factor (TNF). Tumorilytic activity of the CF of Eudrilus eugeniae has not been recorded earlier and therefore suggests further investigations in this animal model.
Authors and Affiliations
M. S. Mohamed Jaabi r 1* , L . S h ams h e e r a l i 2 , M. MD. Yasar 1 , S . S e n t h i l Kuma r 1
Keywords
Related Articles
- EP ID EP86645
- DOI -
- Views 93
- Downloads 0
Microbiology of Genus Arcobacter: A Review
This review updates the knowledge of the genus Arcobacter providing the most recent data on the taxonomy, species diversity, methods of detection, identification and on the role that these microbes play as human and an...
Cervical cancer : Promoter hypermethylation and its reversal
Cervical cancer is one of the most common cancer in women resulting in 0.4% of the total mortality across the world. A number of epigenetic alterations occur during all stages of cervical carcinogenesis including global...
Evaluation of anti-inflammatory activity of the leaf extract of Wrightia tomentosa. Roem & Schult
The methanol: dichloromethane (1:1) extract of the leaves of Wrightia tomentosa (Family: Apocynaceae), a traditional medicine plant was tested for anti-inflammatory activity dextran induced paw edema in rats. The results...
Phytochemical profiling, antimicrobial and cytotoxicity studies of methanolic extracts from Ruta graveolens
Aim of study: Ruta graveolens is found to have potential value in traditional medicine its components are of great interest in medicinal chemistry. It contains many active principles that act as antioxidants and possess...
Isolation and purification of antibacterial protein from Euphatorium odoratum: A seasonal folk medicinal plant
A potent protein with molecular weight of 15.85 kDa exhibited significant antibacterial activity was isolated and purified from leaf extract of Euphatorium odoratum. It is a seasonal girijan folk medicinal plant, of Co...