Experimental In Vitro Mouse and Human Stem Cell Models for Balanced Activity of Oncogenes and Tumor-Suppressor Genes
Journal Title: Journal of Physiology and Pharmacology Advances - Year 2012, Vol 2, Issue 1
Abstract
Taking in consideration many literature data, according which coordinated oncogenes and tumor-suppressor genes functions is of most importance. In this connection, the main idea was balanced activity of oncogenes and tumor-suppressor genes in vitro in laboratory-cultivated normal cells be achieved. Additional copy of oncogene was inserted Dcn1 in mouse embryonic stem cells (mESCs) by their transfection with appropriate recombinant DNA-constructs. On the other hand, in cultivation of separate sub-populations of non-transfected mESCs in the presence of Doxicyclin, suppressed cell proliferation and increased myeloid cell differentiation was observed, probably by activation of genes from STAT-family. After in vitro-co-cultivation of sub-populations of transfected mESCs, containing additionally-inserted copy of oncogene Dcn1 with sub-populations of non-transfected mESCs, treated with Doxicyclin, the results obtained have indicated the safety and preserved normal/non-tumorigenic cell character of the so derived stem cells in vitro, despite of the presence of additional oncogene copy. These data were confirmed by results from similar experiments with analogical human normal trophoblast cells, immortalized by transfection with SV40 recombinant gene vector.
Authors and Affiliations
Sainova I , Vavrek I , Pavlova V , Daneva T , Iliev I , Yossifova L , Gardeva E , Nikolova E
Keywords
Related Articles
- EP ID EP120038
- DOI -
- Views 87
- Downloads 0
Plasma Total Protein, Serum Calcium and Inorganic Phosphate Levels in Nigerian Horses with Natural Piroplasmosis
The objectives of this study were to determine alterations in plasma total protein, serum calcium and phosphorus levels in horses with natural piroplasmosis. Knowledge of these alterations, enable clinicians decide on th...
Study of Possible Colonic Mucosal and Motility Changes in Experimental Acetic Acid Induced Colitis in Male Albino Rats under the effect of Oral Barley Administration in Comparison with Venlafaxine
The current study was conducted on 40 male albino rats subjected to acetic acid 4% induced colitis except the negative control group I .Its scope concerned histological and functional colonic evaluation of pretreatment w...
Experimental Rodent and Human in Vitro-Models for Balanced Activity of Oncogenes and Tumor-Suppressor Genes
Taking in consideration the importance of coordinated oncogenes and tumor-suppressor genes activity in the control of malignancies and death, additional copy of oncogene Dcn1 was inserted in mouse embryonic stem cells (m...
Plasma Lipid Profile in Non-Diabetic Rats Treated with Extracts of Triplochiton Scleroxylon K. Schum
In the view to ascertain any possible disposition of aqueous and 50% ethanol extracts of Triplochiton scleroxylon to cardiovascular diseases, plasma lipid profile and glucose concentration were investigated in nondiabeti...
Prepubertal Induction of Obesity Impaired Reproductive Function in Male Rat at Maturity
The effect of obesity on reproductive function in obese men is much less investigated than in women. In addition, the mechanism of male reproductive outcomes in obesity is unknown. So this study was designed to investiga...