Expression and clinical significance of chemokine receptor in peripheral blood mononuclear cells in patients with primary biliary cirrhosis

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Expression and clinical significance of chemokine receptor in peripheral blood mononuclear cells in patients with primary biliary cirrhosis

Journal Title: Di-er Junyi Daxue Xuebao - Year 2009, Vol 30, Issue 2

Abstract

Objective:To investigate the relationship of chemokine receptor with the development and progression of primary biliary cirrhosis (PBC). Methods: Real-time PCR and flow cytometry were used to examine the mRNA and protein expression of chemokine receptor 1 (CCR1),CCR3 and CCR5 in the peripheral blood mononuclear cells (PBMCs) of 60 patients with PBC,60 patients with hepatitis B-related cirrhosis, and 60 normal controls. Total bilirubin (TBIL) and γ-glutamyltransferase (γ-GT) levels were determined in the patients with PBC and normal controls,and their correlation with chemotactic factors was also analyzed. Results: Both the mRNA and protein expression levels of CCR1,CCR3 and CCR5 in the PBMCs were significantly lower in PBC patients than those in the other two groups (P<0.05), and there were no significant differences between the latter two groups. The mRNA and protein levels of CCR1,CCR3 and CCR5 were significantly lower in patients with stage Ⅲ and Ⅳ PBC than those with stage Ⅰ and Ⅱ PBC. CCR1 protein expression was correlated with TBIL levels in PBC patients(r=- 0.445,P<0.01), but not with γ-GT(r=-0.230,P>0.05). CCR3 protein was not linearly correlated with TBIL level (r=-0.173,P>0.05),but was correlated with γ-GT(r=-0.295,P<0.05). Expression of CCR5 protein was negatively correlated with both TBIL and γ-GT levels(r=-0.531,P<0.01; r=-0.665,P<0.01). Conclusion: CCR1,CCR3 and CCR5 expression is associated with the development and progression of PBC; they may be involved in the regulatory mechanism of PBC,which may cast new lights on the diagnosis and prevention of PBC.

Authors and Affiliations

Zhi-jun HAN

Keywords

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EP ID EP83030
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