Extended-Spectrum -Lactamase-Producing Salmonella Species Isolated from Diarrhoeal Patients in Bangladesh: Characterization and Their Dissemination through Conjugation
Journal Title: Microbiology Research Journal International - Year 2015, Vol 6, Issue 1
Abstract
Objective: Infections with Salmonella resistance to extended-spectrum cephalosporins threaten the efficacy of drugs for treating samonellosis. So the aim of this study was to characterize the ESBL (Extended Spectrum β-lactamase)-producing Salmonella species. Methods: The ESBL-producers were extensively characterized using antibiogram, double disc diffusion synergy test, plasmid profiling, PCR, pulsed-field gel electrophoresis (PFGE) and conjugation experiments. Results: Of the 200 Salmonella strains, n=8 were found to be ESBL-producers and these belonged to only two serogroups, Salmonella Group B (n=4) and Salmonella Group G (n=4). Most of the ESBL-positive strains were found to be resistant to third and fourth-generation cephalosporins and monobactams. Plasmid profiling indicated that n=6 and n=2 of the ESBL-producing strains harbored a 62-MDa and a 90-MDa plasmid, respectively. The PCR analysis revealed that blaTEM (β-lactamase Enzyme TEM Producing Gene) (n=6) was most predominant gene, followed by the blaOXA (β-lactamase Enzyme OXA Producing Gene) (n=4), blaSHV (β-lactamase Enzyme SHV Producing Gene) (n=2) and blaCTX-M-1 (β-lactamase Enzyme CTX-M-1 Producing Gene) (n=2) genes. Fifty percent (n=4) of the strains were positive for the int1 gene. The PFGE analysis revealed that almost similar clones were disseminated within the ESBL-producing strains and the non-ESBL-producing strains. The conjugation study revealed that the 62-MDa plasmid was transferred to E. coli K-12 and contained the blaTEM, blaOXA, blaCTX-M-1 and int1 genes. Conclusion: The emergence of ESBL-producing Salmonella is of great concern and horizontal gene transfer plays an important role in the spread of ESBL.
Authors and Affiliations
Afroza Parvin, Md. Mahmudul Hasan, Kaisar Ali Talukder, Israt Jahan Azmi, Md. Rakib Hasan
Keywords
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- EP ID EP354745
- DOI 10.9734/BMRJ/2015/11967
- Views 79
- Downloads 0
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