Genetic diversity of Lolium Multiflorum accessions using ISSR molecular markers
Journal Title: International Journal of Farming and Allied Sciences - Year 2013, Vol 2, Issue 1
Abstract
Genetic diversity based on different markers, plays a key role in breeding programs and one of the most important criteria for selection of Parents. In this study the genetic diversity among 13 accessions of Lolium Multiflorum was investigated using 9 ISSR primers. These primers could identify 66 bands. The average of bands was 7.36 for each primer. Average PIC in the used primers was 0.404 that the highest amount of PIC related to primers IS11, IS14 and IS16 and primers IS5, IS11 and IS13 with the lowest value of PIC don’t have ability in the separation of accessions. A desirable polymorphism between genotype was observed based on ISSR markers, which the primers of IS9, IS10, IS11, IS14 and IS16 were determined for genetic variation study in Lolium Multiflorum as desirable primers. Also, cluster analysis and principal coordinate analysis using Dice similarity coefficients were calculated and dendrogram was drawn using the UPGMA for 13 accessions. Grouping of accessions indicated that genetic variations do not agreement with the geographical distribution of accessions. The coordinate analysis was performed using of the similarity matrix and confirmed the results of cluster analysis.
Authors and Affiliations
Soosan Abbaszade, Ali Ashraf Jafari, Hooshmand Safari and Hooman Shirvani
Keywords
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