Genetic stability among in vitro eggplant clones induced by different plant growth regulators
Journal Title: Biotecnología Vegetal - Year 2019, Vol 19, Issue 2
Abstract
Many factors may influence the genetic stability of cloned in vitro plants, among which are the genotype and the regenerative process induced by plant growth regulators. The resulting somaclonal variations may be useful for breeding projects, but may be detrimental to germplasm conservation. The objective of this work was to evaluate the genetic stability of Solanum melongena cv. Florida Market clones, obtained in response to different plant growth regulators. For the production of clones, leaf explants were used from commercial seeds in vitro germinated. The explants were inoculated in Murashige and Skoog medium supplemented with different plant growth regulators at pre-defined concentrations. The DNA was extracted by the CTAB method from leaves of complete plants obtained by somatic embryogenesis induced by naphthaleneacetic acid (NAA) or indirect organogenesis induced by benzylaminopurine (BAP) or thidiazuron (TDZ). For the RAPD, 117 DNA samples were amplified by ten decamer primers and 49 specific bands were selected among the products for the comparative study. A total of 5733 fragments were obtained, with a rate of 5.37% polymorphism. NAA induced clones showed no polymorphism. The highest rate of polymorphism was observed in BAP induced clones (14.28%). Two RAPD primers were identified as markers for monitoring the genetic stability of eggplant. The polymorphic pattern was observed only in clones originating from indirect organogenesis. These results indicate the usefulness of a monitoring protocol for studies using in vitro cloned eggplants.
Authors and Affiliations
Ana Paula Mançano, Bianka de Oliveira Soares, Renata de Oliveira Garcia, Elisabeth Atalla Mansur, Rachel Fatima Gagliardi
Keywords
Related Articles
- EP ID EP698028
- DOI -
- Views 66
- Downloads 0
Effect of two brassinosteroid analogues on the induction and regeneration of callus from Ipomoea batatas
With the purpose of studying, in different clones, the effect of two similar brassinoesteroids in the induction of callus and the regeneration of the same ones, tuberous roots belonging to the clones Cemsa 78-354, Inivit...
In vitro regeneration of Solanum pimpinellifolium plants L. from leaf explants
Tomato is a species of agricultural importance. Besides, it is a source of vitamins, minerals and antioxidant compounds. Therefore it is essential to obtain varieties resistant to diseases. Solanum pimpinellifolium L. ‘...
Effect of the culture filtrate of Pseudocercospora fijiensis Morelet on adventitious buds of cultivars of Musa spp.
The obtaining of bananas tolerant or resistant to the Black Sigatoka (Mycosphaerella fijiensis Morelet) is an imperious need since 1991 with its apparition in our country. The effect of the culture filtrate was studied...
Filamentous fungi associated with Bambusa plantations vulgaris var. Schrader vulgaris ex Wendland
Bambusa vulgaris var. vulgaris Schrader ex Wendland is a species of a great economic importance and in the conservation of soils. Nevertheless, little information exists on the microbial communities associated to its pl...
Determination of the minimal inhibitory concentration of Ammonium glufosinate in organogenic calli of Phaseolus vulgaris cv. ‘CIAP 7247F’
An efficient selection system is necessary for distinguishing transformed cells of the untransformed tissue. This study aimed to determine the minimum inhibitory concentration of the herbicide Ammonium glufosinate in o...