HPLC Method Development for Glucosamine Sulphate and Diacerein Formulation
Journal Title: Journal of Pharmacy Research - Year 2010, Vol 3, Issue 2
Abstract
A simple, specific, sensitive, and rapid high performance liquid chromatography (HPLC) method for the determination of Glucosamine sulphate and Diacerein for assay was developed. Glucosamine and Diacerein were baseline separated and quantitated on C8 reversed phase column (4.6×250mm, 5.0µm), using a mobile phase composed of a phosphate buffer-acetonitrile (55:45v/v, pH 3.0) delivered at a flow rate of 0.6mL/min, and with UV detection ( l excitation = 195nm). The method was proven to be linear over a Glucosamine concentration range of 84 to 504µg/mL with a mean correlation coefficient of 0.9999 and a Diacerein concentration range of 5.6 to 33.6µg/mL with a mean correlation coefficient of 0.9998.
Authors and Affiliations
Useni Reddy Mallu1 K. Hussain Reddy1, Varaprasad Bobbarala2* and Somasekhar Penumajji3
Benzimidazole compounds: As antimicrobial agents
Heterocyclic compounds exhibited remarkable pharmacological activities. Literature indicates that compounds of pyrimidine, pyridine, benzimidazole nucleus have wide range of therapeutic uses such as antitubercular, anti...
Microwave Assisted Organic Synthesis: An Overview
Microwave heating refers the use of electromagnetic waves ranges from 0.01 m to 1 m wavelength of frequency 30 GHz to 0.3 GHz to generate heat in the material. These microwaves lie in the region of the electromagnetic sp...
Cervical cancer : Promoter hypermethylation and its reversal
Cervical cancer is one of the most common cancer in women resulting in 0.4% of the total mortality across the world. A number of epigenetic alterations occur during all stages of cervical carcinogenesis including global...
Synthesis and antifungal screening of fluorinated benzothiazolo linked imidazole compounds
4-Fluoro-3-chloroanilline treated with Potassium thiocyanate in presence of Glacial acetic acid and bromine was converted into 2-amino-6-fluoro-7- chlorobenzothiazole, resulting into 2-amino benzothiazole. The synthesize...
Management of hypertension in pregnancy: A review
Hypertension in pregnancy is the most common cause of maternal death, with a risk of approximately 10 deaths / million pregnancies in the UK. Hypertension in pregnancy is also the most common cause of stillbirth and neon...