HPLC Method Development for Glucosamine Sulphate and Diacerein Formulation
Journal Title: Journal of Pharmacy Research - Year 2010, Vol 3, Issue 2
Abstract
A simple, specific, sensitive, and rapid high performance liquid chromatography (HPLC) method for the determination of Glucosamine sulphate and Diacerein for assay was developed. Glucosamine and Diacerein were baseline separated and quantitated on C8 reversed phase column (4.6×250mm, 5.0µm), using a mobile phase composed of a phosphate buffer-acetonitrile (55:45v/v, pH 3.0) delivered at a flow rate of 0.6mL/min, and with UV detection ( l excitation = 195nm). The method was proven to be linear over a Glucosamine concentration range of 84 to 504µg/mL with a mean correlation coefficient of 0.9999 and a Diacerein concentration range of 5.6 to 33.6µg/mL with a mean correlation coefficient of 0.9998.
Authors and Affiliations
Useni Reddy Mallu1 K. Hussain Reddy1, Varaprasad Bobbarala2* and Somasekhar Penumajji3
Keywords
Related Articles
- EP ID EP83418
- DOI -
- Views 189
- Downloads 0
Formulation and evaluation of lovastatin fast dissolving tablets using newer super disintegrants
The objective of this study was to improve the solubility and dissolution rate of a poorly water-soluble hydrophobic drug, Lovastatin with Polyethylene glycol-6000 as a carrier, by solid dispersion techniques. The disper...
Antidiabetic and Antioxidant Effect of Ethanolic Root Extract of Boerhaavia diffusa in Streptatozocin-Induced Diabetic Rats
The ethanolic root extract of Boerhaavia diffusa have potent antidiabetic activity that reduces blood sugar level in Streptatozocin-Induced diabetic rats. The present study was carried out to investigate ntihypergl...
Determination of LD50 and ED50 by dose response relationship and assessment of toxicological and non toxicological behaviour of Ipomoea hederacea
The present study was designed to evaluate the toxic and non toxic profile of Ipomoea hederacea (Convolvulaceae). The results of oral toxicity study was observed in mice at the doses of 300, 200, 100, 50, 25, 10, 1, 0.0...
Bis-Schiff Bases of Isatins: A New Class of Antioxidant
Twenty seven (27) derivatives of bis-Schiff bases of isatins 1-27 were screened for their antioxidant potential. Out of twenty seven (27), four compounds exhibited a varying degree of superoxide anion radical scavenging...
Cervical cancer : Promoter hypermethylation and its reversal
Cervical cancer is one of the most common cancer in women resulting in 0.4% of the total mortality across the world. A number of epigenetic alterations occur during all stages of cervical carcinogenesis including global...