Immunocytochemistry(ICC) on FNAC of breast carcinoma lesions to study ER, PR and Her2/neu status with immunohistochemistry(IHC) correlation
Journal Title: Medpulse International Journal of Pathology - Year 2018, Vol 7, Issue 2
Abstract
Background: Immunocytochemistry (ICC) is an ancillary laboratory technique used to visualise the localisation of specific antigens in cells by use of a specific primary antibody that binds to it. ICC differs from immunohistochemistry (IHC) in that the former is performed on sample of intact cells of smears whereas the latter is performed on tissue sections. Estrogen receptors (ER), Progesterone receptors (PR) and Her2 receptor status in breast carcinoma plays an eminent role in the patient management. ICC technique displays merits over IHC along with producing comparable results. Aims and Objectives: To evaluate the reliability of ER, PR, and Her2/neu receptor status by ICC on FNAC smears by comparing the results with IHC on tissue sections and calculating the sensitivity, specificity, positive predictive value, negative predictive value and concordance between ICC and IHC. Design-A Two year Longitudinal Prospective Study Materials and Methods: FNAC smears of 100 female breast carcinoma patients were stained with HandE stain, checked for cellularity, marked, destained and then used for performing ICC. Corresponding patients MRM specimen’s tissue sections were taken for IHC. ICC and IHC both were performed by the peroxidase- antiperoxidase technique. ER, PR positivity was denoted using Allred scoring system. ASCO/CAP guidelines was used for Her2/neu grading. Results of both ICC and IHC were correlated. Statistical Analysis used- Chi-Square Tests, Results and Conclusion: The study showed a good concordance of 89%, 92% and 94% for ER, PR and Her2 receptors respectively between the ICC and IHC findings. ICC can be a paramount prognostic and therapeutic adjunct in the management of the patients with breast carcinoma due to its rapid turnaround time with results as good as that of gold standard IHC.
Authors and Affiliations
Suchita Mishra, Pravina M Santwani
Keywords
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