Impact of systemic cryotherapy on selected enzymes, glutathione and serum total protein levels in healthy young males
Journal Title: Antropomotoryka. Journal of Kinesiology and Exercise Sciences - Year 2015, Vol 25, Issue 70
Abstract
Study aim. Determining the impact of systemic cryotherapy on the levels of AChE (acetylcholinesterases), G-6-PD (glucose-6-phosphate dehydrogenases), GSH (glutathione), plasma proteins and occult Hb (occult hemoglobin) in young healthy men.Study group. The study group comprised of the 10 healthy men (untrained) aged 23–24 years (23.4 ± 0.52), who underwent cryotherapy treatments (treatment time 3 min, chamber temperature of –120°C, 10 treatment sessions – 5 times a week). The average body height was 179.2 ± 6.4 cm, weight 79.6 ± 8.8 kg, BMI 23.4 ± 2.6 kg/m2. In order to analyze the levels of AChE, G-6-PD, GSH, occult hemoglobin and plasma proteins, venous blood was collected from the study participants three times. The first test (control) took place two months before cryotherapy, the second on the day of beginning treatment and the third test after a series of 10 treatments.Methodology. Measurements were taken using the Helios Beta Z OP Spectro-Lab spectrophotometer, the VisionPro 4.10 Thermo Electron UV-Visible Spectroscopy program. To measure the concentration of AChE [U/gHb] and G-6-PD [U/gHb], the method proposed by Beutler (1986) was used. Plasma hemoglobin concentration [g/100 ml] was determined using the method by Chanazin (1989). The concentration of reduced glutathione GSH [mmol/gHb] was determined on the basis of the method developed by Ellman (1970). The Biuret method was used for the determination of total protein [g/l].Results. Analyzing the average values of AChE [U/gHb], G-6-PD [mmol/gHb] and occult Hb. [g/dl] before and after the usage of systemic cryotherapy, no statistically significant differences were found. However, an increase in total protein concentration [g/l] and a decrease in GSH concentration [mmol/gHb] were found in the men who underwent cryotherapy in relation to the measurement taken two months before starting the treatment.Conclusions. Systemic cryotherapy does not cause any side effects in young, healthy men. Regular usage of cryotherapy treatments may affect the levels of enzyme indicators – total protein (concentration increase) and glutathione (concentration reduction) in the blood.
Authors and Affiliations
Bartłomiej Ptaszek, Aneta Teległów, Jacek Głodzik, Jakub Marchewka
Keywords
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