In vitro conservation of Ipomoea batatas (L.) Lam cultivars by minimum growth with the use of mannitol (2019)
Journal Title: Biotecnología Vegetal - Year 2019, Vol 19, Issue 1
Abstract
ABSTRACT In vitro conservation of Ipomoea batatas (L.) Lam allows the exchange of germplasm and its availability for breeding programs. The objective of this work was to determine the effect of mannitol and abscisic acid in the conservation through minimum growth of I. batatas cultivars from INIVIT Germplasm Bank. Treatments included abscisic acid (ABA) (5 and 10 mg l -1 ) and mannitol (1.0, 1.5 and 2.0%) in a basal MS culture medium, the combination of ABA (10 mg l -1 ) and mannitol (1.0, 1.5 and 2.0%). As controls were used the MS basal culture medium and MS with sorbitol (1.0%) and glucose (1.0%). In the cultivars ‘Cautillo’ and ‘INIVIT BS 16-2006’ the best results of survival, growth decrease and green but small leaves were obtained in the treatments that contained the basal culture medium with mannitol (10, 15 and 20%) . Growth of the explants was not achieved in culture media with abscisic acid. The MS culture medium with 2 mg l -1 of thiamine, 100 mg l -1 of myo-inositol and 1.0% of mannitol, allows the in vitro conservation of sweet potato cultivars between six and eight months.
Authors and Affiliations
Aymé Rayas Cabrera, Jorge López Torres, Víctor R Medero Vega, Milagros Basail Pérez, Arletys Santos Pino, Yenisey Gutiérrez Sánchez
Keywords
Related Articles
- EP ID EP697989
- DOI -
- Views 73
- Downloads 0
In vitro antifungal activity of bacteria against Mycosphaerella fijiensis mediated by diffused and volatile metabolites
Antagonistic microorganisms do not have a unique mode of action. Multiplicity of these is an important feature for selection as biological control agents. Black Sigatoka is considered the foliar disease with most economi...
New somaclone of viand plantain (Musa AAB) obtained by biotechnological techniques
Results obtained from Plant Breeding Programs on Musa spp. In the tissue culture Laboratory at INIVIT since 1987 is presented. Procedures described by IAEA with some modifications for mutation induction through in vitro...
Reduction of negative environmental impact generated by residues of plant tissue culture laboratory
The research is based on the activity developed by teaching and research laboratories for biotechnology purposes with an environmental approach to determine potential contamination risk and analyze the residuals genera...
Expression analysis of three phenylpropanoid-related genes during Musa spp.- Mycosphaerella fijiensis interaction and in response to ethephon
Phenylpropanoid pathway is one of the best-studied, most engineered and frequently induced by pathogens or pathogen elicitors. Patterns of expression of chalcone synthase (CHS), flavonoid 3’5' hydroxylase-like (F3’5’H-...
Induction of somatic embryogenesis in Cocos nucifera L. from immature inflorescences
Including all the methods of vegetative multiplication, the somatic embryogenesis is the most promising for coconut multiplication. Immature inflorescences of Coppery Malay from different ages trees were used. Three con...