In vitroantisicklingactivity of the aqueous extract of a combination of three plants: Jatropha grossypiifolia, Justicia secunda and Parquetina nigrescens from East Côte d’Ivoire.
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 6
Abstract
Sickle cell disease results in hemolytic anemia, vaso-occlusive crisis and susceptibility to infections. In Côte d’Ivoire, its prevalence is estimated at more than 14%. Patients use traditional pharmacopoeia with medicinal plants to treat themselves. The objective of this study was to verify the effectiveness of the aqueous extract of the composition of three medicinal plants (Jatropha grossypiifolia, Parquetinanigrescens and Justicia secunda) used in the treatment of sickle cell disease at East of the Côte d’Ivoire. The Emmel method taken up by Imaga was used to study the reversibility of sickle cells, as a function of the incubation time by the aqueous extract relative to the controls (NaCl and phenylalanine) on blood samples from SS homozygous patients. Concentrations of 10, 5, 0.5 and 0.05 mg / mL of the aqueous extract were contacted with sickle cells after pretreatment sickling cells with 2% sodium met bisulfite solution. Sickle cell determination was performed every 30 minutes for 120 minutes using a SQA Vision microscope. The extract showed activity on sickle cell reversion. The analysis of the histogram shows a decreasing rate according to the positive control Phenylalanine. This activity is dose-dependent (65, 70, 75 and 80%) vs Concentrations of 0.05;0.5;5 and 10 mg /mL. The reversion rate is 80% for the aqueous extract. Phytochemical screening showa correlate between the phenolic compounds with the antisickling activity of the aqueous extract plant combination. The use of the aqueousextract threeplant combination in a traditional environment would be justifiedin East Côte d’Ivoire.
Authors and Affiliations
Akakpo-Akue J. , Kplé Tatiana . K. M. , Yapo -Crezoit . A. , Fofié Y. , Kra A. M. , Tolo D. A, N’Guessan J. D.
Keywords
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