ISOLATION AND IDENTIFICATION OF RNASE A FROM TESTIS THROUGH HPLC AND CHROMATOFOCUSSING
Journal Title: Indo American Journal of Pharmaceutical Research - Year 2017, Vol 7, Issue
Abstract
Abstract From the previous studies it was clear that RNase A is present in pancreas and our new Finding includes that RNase that is similar to RNase A was also present in testis along with other RNases in the tissue. In chromatofocussing the peak was observed in fraction 5 of PI 9.6 and in HPLC it was present in all fractions of the column. The aim of present work is to isolate and identify RNase A through HPLC and chromatofocussing. From the results we conclude that RNase A is present in testis in addition to its presence in epidydymis .Future perspectives include finding of other RNases present in the testis and also further evaluation by other methods.
Authors and Affiliations
EswariBeeram. , Kamala Katepogu. , ThyagarajuKedam*
ASSESSMENT OF ANTIEMETIC POTENTIAL OF CRUDE EXTRACT OF VIGNA TRILOBATA (LINN.) AGAINST DIFFERENT EMETOGENIC STIMULI; AN IN VIVO STUDY
Vigna trilobata (Linn.) is used in traditional system for the treatment of many ailments in Asia. The present study is an attempt to evaluate the antiemetic activity of V. trilobata (50 and 100 mg/kg of body weight) by u...
Recent Trends in Screening and Evaluation Methods of Anticancer Drugs
Cancer is one of the major life threatening diseases worldwide. The available anticancer drugs have distinct mechanisms of action which may vary in their effects on different types of normal and cancer cells. Screening m...
A COMPREHENSIVE REVIEW ON FUTURE PROSPECTS FOR FAST DISSOLVING ORAL FILMS
The ultimate goal of any drug delivery system is the successful delivery of the drug to the body, however patient compliance must not be overlooked. Oral drug delivery is the most widely utilized route of administration...
RECENT PROSPECTIVE ON SOLID LIPID NANOPARTICLES AND ITS APPLICATION- A REVIEW
Abstract Solid Lipid Nanoparticles are at the forefront of the rapidly developing field of nanotechnology with several potential applications in drug delivery and research. Due to their unique size dependence properties,...
A HIGH THROUGHPUT LIQUID CHROMATOGRAPHY TANDEM–MASS SPECTROMETRY METHOD FOR THE QUANTIFICATION OF NAPROXEN SODIUM IN HUMAN PLASMA: APPLICATION TO PHARMACOKINETIC STUDY
Abstract Background: A LCMS-MS method has been validated for the determination of naproxen using ketoprofen as an internal standard. Methods: Protein precipitate method was used to extract both analytes and internal stan...