Lactobacillus AS PRODUCERS OF EXTRACELLULAR TANNASE
Journal Title: Biotechnologia Acta - Year 2018, Vol 10, Issue 5
Abstract
The aim of the work was to find strains of lactic acid bacteria capable to synthesize extracellular tannase enzyme − the key enzymehe hydrolyzing tannins which are plant food constituent. One of the main product of tannins hydrolyzis is gallic acid- the compound with proven antioxidant and onco-protective properties. As a result of lactobacteria screening, two biocompatible strains of lactic acid, namely L. rhamnosus LB3 and L. delbrueckii subsp. delbrueckii with a high level of enzyme productivity, were selected. The maximum accumulation of tannase, corresponding to 0.031 ± 0.002 U/ml for L. rhamnosus LB3 and 0.03 ± 0.002 U/ml for L. delbrueckii subsp. delbrueckii, was observed after 48 h of cultivation. Both strains showed rapid growth and performance of tannase in MRS medium in the presence of glucose or lactose as a carbon source. It was shown that gallic acid, which was a necessary component of the medium as a target enzyme inducer, did not affect the accumulation of lactobacilli biomass. The selected strains are of interest as producers of a bicomponent probiotic with antioxidant properties and require further investigation.
Authors and Affiliations
L. Oriabinska, O. Dziuba, O. M. DUGAN
Keywords
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- EP ID EP423783
- DOI 10.15407/biotech11.05.065
- Views 144
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