Method Development and Validation of Piperacillin and Tazobactum in Pharmaceutical dosage form by using RP-HPLC Method
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2017, Vol 5, Issue 5
Abstract
A simple and selective LC method is described for the determination of Piperacillin and Tazobactum in tablet dosage forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixture of 50 volumes of Triethylamine and 50 volumes of Acetonitrile with detection of 226 nm. Linearity was observed in the range 5-15 µg /ml for Piperacillin (r2 =0.996) and 10-30 µg /ml for Tazobactum (r2 =0.997) for the amount of drugs estimated by the proposed methods was in good agreement with the label claim. The proposed methods were validated. The accuracy of the methods was assessed by recovery studies at three different levels. Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods and can be used for routine analysis of pharmaceutical dosage form.
Authors and Affiliations
Pendela Kavitha, A. Sirisha, P. Parthiban, M. Vinod Kumar
Keywords
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