Modified method of JSCC candidate reference procedure for determination of serum pancreatic lipase activity measurement
Journal Title: Journal of Analytical Bio-Science - Year 2013, Vol 36, Issue 2
Abstract
The present JSCC candidate reference procedure for determination of serum pancreatic lipase activity measurement must be modified to measure the sample blank for each specimen. Although this method is now regarded as one of the primary reference measurement procedures in IFCC, measurement of the sample blank has been pointed out as a serious drawback of the method. In order to solve the problem, a modified method without the sample blank has been developed and here its effectiveness was studied. As a result, the average sample blank value and SD in terms of lipase activity was 2.6U/L and 5.3U/L, respectively, compared with the present method of corresponding value as 1.6U/L and 16.5U/L among 274 human sera. As a whole, the validity of the modified method was confirmed as SD of sample blank values decreased one third of that of the present method. However, there remained some specimens with sample blank values of 40U/L to the maximum in a particular case even in the modified method. The future challenge is what the cause of the results is.
Authors and Affiliations
Yoshiaki Iizuka
Keywords
Related Articles
- EP ID EP109310
- DOI -
- Views 87
- Downloads 0
Management of hepatitis B
Hepatitis B virus (HBV) infection is a global public health problem. HBV has been classified into nine genotypes (A to J) by a complete nucleotide sequence. The prevalence of specific genotypes varies geographically. The...
Whole blood concentration of thiamine diphosphate requires correction for red blood cell count in the nutritional assessment of thiamine status
This study examined whether the whole blood (WB) concentration of thiamine diphosphate (TDP) needed to be corrected for hemoglobin concentration (Hb) or red blood cell (RBC) count. TDP levels in whole blood (WB TDP) and...
Monomeric sarcosine oxidase acts on both L- and D-substrates
The stereoselectivity of monomeric sarcosine oxidase (mSox), an enzyme used for creatinine determination, was assayed by using N-methylalanine and proline. As a result, mSox reacted to both L- and D-substrates, although...
Clinical state of acute myocardial infarction with increased phosphoglucomutase activity: The relationship between phosphoglucomutase activity and acute thrombosis
Phosphoglucomutase (PGM) is a key enzyme in cellular glucose utilization and energy homeostasis. We previously found that patients with acute myocardial infarction (AMI) showed high PGM activity, especially those with ST...
Medical laboratory and international standards
With the increased globalization of the economy, that of the medical field (including the medical laboratory) becomes an active participant in the structure of the world economy. As patients and specimens now move acros...