Modified method of JSCC candidate reference procedure for determination of serum pancreatic lipase activity measurement
Journal Title: Journal of Analytical Bio-Science - Year 2013, Vol 36, Issue 2
Abstract
The present JSCC candidate reference procedure for determination of serum pancreatic lipase activity measurement must be modified to measure the sample blank for each specimen. Although this method is now regarded as one of the primary reference measurement procedures in IFCC, measurement of the sample blank has been pointed out as a serious drawback of the method. In order to solve the problem, a modified method without the sample blank has been developed and here its effectiveness was studied. As a result, the average sample blank value and SD in terms of lipase activity was 2.6U/L and 5.3U/L, respectively, compared with the present method of corresponding value as 1.6U/L and 16.5U/L among 274 human sera. As a whole, the validity of the modified method was confirmed as SD of sample blank values decreased one third of that of the present method. However, there remained some specimens with sample blank values of 40U/L to the maximum in a particular case even in the modified method. The future challenge is what the cause of the results is.
Authors and Affiliations
Yoshiaki Iizuka
Keywords
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