Monitoring the persistence of a genetically modified strain of the Azorhizobium caulinodans in the rhizosphere of Triticum aestivum L.
Journal Title: Biotecnología Vegetal - Year 2013, Vol 13, Issue 1
Abstract
A protocol was developed to monitor persistence and spread of a genetically modified strain of the growth promoting diazotroph Azorhizobium caulinodans used to inoculate field grown wheat. The protocol was used to identify an endogluconase (egl) overproducing strain of A. caulinodans isolated from inoculated soils. A detection limit of 2500 cfu g -1 and 10 8 –10 10 cfu g -1 of soil was determined using the BIOLOG ® fingerprinting method and PCR technique, respectively. Since none of the tested samples were positive for the bacteria or the Egl 1 gene construct, it was concluded that A. caulinodans ORS 571 pGV910-C1 did not persist or spread in any of the tested field locations. This result may be due to low soil temperatures and competition of indigenous microorganisms: environmental factors that were not favorable for the diazotroph to thrive in the test locations. The application of different inoculation methodologies, as well as the study of other rhizobial genera for the inoculation of wheat in further experiments, is strongly recommended.
Authors and Affiliations
Jan Jacek Slaski , Daniel Joseph Archambault , John Vidmar , Carlos José Bécquer , Anthony Anyia
Keywords
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