A high frequency microcloning protocol for subsequent cryopreservation in Kaempferia galanga L.: An endangered, over exploited medicinal plant in pharmaceutics
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2014, Vol 4, Issue 12
Abstract
: An efficient in vitro propagation protocol was standardized in K. galanga, wherein shoot cultures were raised from rhizome with axillary bud explants in Murashige and Skoog (MS) medium supplemented with different hormonal regimes. Maximum 10.6±0.83 multiple shoots per explants were obtained in MS medium supplemented with 4.0 mgl-16-benzyl adenine (BA) along with 1.0 mgl -1each of α-naphthaleneacetic acid (NAA)and kinetin. Subsequent 2-3 subculture passages enhanced the shoot multiplication rate to 3-4 times. The multiple shoots thus produced were having individual root system and elongated substantially when kept in the same medium for 4-6 weeks. There was no need of elongation and root induction phase and this two-step multiplication procedure reduced the period of plantlet producton compared to the earlier protocols. The regenerated plants after a short hardening phase got established in the field at 80-90% efficiency and their genetic uniformity was confirmed through ISSR analysis. The protocol thus standardized is an efficient method for rapid propagation of this high value medicinal species by which at least 30 shoots per explants can be produced within 12 weeks duration. It offers the possibility of raising physiologically uniform plants for easy dissection of shoot tip meristems which can be efficiently utilized for subsequent long-term conservation through cryopreservation.
Authors and Affiliations
T. S. Preetha # , A. S. Hemanthakumar, P. Padmesh, P. N. Krishnan
Keywords
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