A NEW METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF ELETRIPTAN BY USING RP-HPLC METHOD
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 5
Abstract
ABSTRACT A simple, rapid, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed for estimation of eletriptan in tablet dosage form. Chromatographic separation was carried out by using mobile phase 0.02M potassium dehydrogenate Phosphate buffer (ph 3.0) : Acetonitrile (50:50 v/v, PH-3.0 adjusted with Orthophosphoric acid ) on Hypersil, Symmetry C8 150×4.6mm, 5m at flow rate 0.8ml/min in isocratic mode and effluents are monitored at 221 nm. The retention time for eletriptan was found to be 2.45 min respectively and calibration curves were linear over concentration from 10-50μg/ml. The method was validated for accuracy, precision, specificity, linearity, system suitability. LOD and LOQ were 0.0258μg/ml and 0.0897μg/ml for eletriptan. The percentage recovery of eletriptan was found to be 98-102%.The developed method was fast, accurate, precise, and successfully applied to estimate the amount of eletriptan in bulk sample and tablet dosage form so it can be used for quality control department. Keywords: Eletriptan, RP-HPLC, Hypersil column.
Authors and Affiliations
S. Sirish Chandra*, T. Priyanka, Naga Sowjanya, A. Ajitha, Uma Maheswara Rao
Keywords
Related Articles
- EP ID EP164581
- DOI -
- Views 127
- Downloads 0
AN OVERVIEW OF ELECTRON PROBE MICROANALYSIS
ABSTRACT Electron Probe Microanalysis (EPMA) is an analytical technique that is used to establish the composition of small areas on specimens. It’s one of the several particle-beam techniques These analytical te...
SPECTROPHOTOMETRIC ESTIMATION OF DRUGS USING NBROMO SUCCINAMIDE AND AMARNTH DYE COUPLE
ABSTRACT Simple, specific, accurate and precise UV–visible spectrophotometric methods have been developed for the estimation of five drugs viz., Dobutamine hydrochloride, Duloxetine hydrochloride, Ondansetron hyd...
DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR QUANTITATIVE ESTIMATION OF CEFTAROLINE FOSAMIL IN BULK AND INJECTION FORM
ABSTRACT Two simple, sensitive, specific, spectrophotometric methods have been developed for the determination of ceftaroline fosamil in bulk and Injection forms. The λmax of ceftaroline fosamil was found to be 2...
Spectrophotometric Estimation of Promethazine Hydrochloride In Bulk and Pharmaceutical Formulations
ABSTRACT Two simple, accurate, rapid and sensitive methods (A and B) have been developed for the estimation of Promethazine Hydrochloride in its pharmaceutical dosage form. The method A and B are based on the for...
ASSAY METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF PAROXETINE AND CLONAZEPAM BY RP- HPLC
ABSTRACT A simple, precise, rapid, specific and accurate reverse phase high performance liquid chromatography method was developed for simultaneous estimation of Paroxetine and Clonazepam in pharmaceutical dosage...