A VALIDATED SIMULTANEOUS ESTIMATION OF DOXYLAMINE SUCCINATE AND PYRIDOXINE HYDROCHLORIDE BY UV – SPECTROPHOTOMETRIC METHOD IN BULK AND FORMULATION
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 2
Abstract
ABSTRACT A simple and rapid UV – Spectrophotometric method was developed and validated for the simultaneous estimation of Doxylamine succinate and Pyridoxine HCl in combined dosage forms. The said combination is used in treating morning sickness associated with pregnancy. For the simultaneous estimation of both drugs, 260 nm and 285 nm were selected as wavelength of analysis. The linearity range was taken 5-50μg/ml for Doxylamine and 2-18µg/ml for Pyridoxine which showed correlation coefficient of 0.9995 and 0.9998 respectively. The method was validated for accuracy and precision and % RSD was found to be within the acceptable limits. The percentage recoveries for both drugs were in the range of 95.5% – 98.33%. The LOD and LOQ of both drugs indicate that the method is sensitive. The assay of the method was in good agreement with the standards and enables the method to be adapted for routine analysis of the both drugs in combined dosage forms. Keywords: Doxylamine succinate, Pyridoxine Hydrochloride, Simultaneous equations, Morning Sickness.
Authors and Affiliations
V. Rajani sekhar*, I. Sowkar Baig, R. Amulya, P. Nagarjuna
Keywords
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