DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHODFOR SIMULTANEOUS ESTIMATION OF ATORVASTATIN AND OLMESARTAN IN PHARMACEUTICAL DOSAGE FORM
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 6
Abstract
ABSTRACT A simple, precise, rapid, specific and accurate stability indicating reverse phase high performance liquid chromatography method was developed for simultaneous estimation of Atorvastatin (ATV) and Olmesartan (OLM) in pharmaceutical dosage form. Chromatographic separation was performed onAgilent eclipse XDB C8 (150x4.6mm, 5μ)column, with mobile phase comprising of mixture of buffer (pH3.25, adjusted with ortho phosphoric acid), and methanol in the ratio of 60:40v/v, at the flow rate 0.8 ml/min. The detection was carried out at 287 nm. The retention times of OLM and ATV were found to be 2.47 and 3.77 mins respectively with a run time of 6 mins, theoretical levels for OLM and ATV were 6248 and 4867 respectively, with a resolution of 7.57. As per ICH guidelines the method was validated for linearity, accuracy, precision, limit of detection and limit of quantitation, robustness and ruggedness. Linearity of OLM was found in the range of 120-360 μg/mL and that for ATV was found to be 60-180 μg/mL. The correlation coefficient for OLM and ATV were 1.000 and 0.999 respectively. The LOD values for OLM and ATV were 2.845 and 2.927 μg/mL respectively. The LOQ values for OLM and ATV were and 9.486 and 9.756 μg/mL respectively. This demonstrates that the developed method is simple, precise, rapid, selective, accurate and reproducible for simultaneous estimation of OLM and ATV tablet dosage form. Keywords: Atorvastatin (ATV), Olmesartan(OLM), RP-HPLC, Validation, Forced Degradation Studies.
Authors and Affiliations
Ranjeet Kumar V*, Sai Malakondaiah D, Ajitha A, Uma Maheshwara Rao V
Keywords
Related Articles
- EP ID EP90736
- DOI -
- Views 246
- Downloads 0
IN VITRO CONTROL OF SELECTED PATHOGENIC ORGANISMS BY METHANOLIC-AQUA EXTRACT OF ACANTHOSPERMUM AUSTRALE LEAVES
ABSTRACT The current study was done to analyse the antibacterial activity of the methanolic- aqua extract of Acanthospermum australe. From the study, the plant was found to inhibit only Bacillus cereus (20.00± 0...
FORMULATION AND EVALUATION OF ENTERIC COATING TABLETS BY WET GRANULATION METHOD
ABSTRACT Serratiopeptidase is derived from bacteria belonging to genus Serratia. Serratiopeptidase tablets used in the treatment of viral diseases and hepatitis. Serratiopeptidase were formulated using HPMC phth...
SPECTROPHOTOMETRIC ESTIMATION OF DRUGS USING NBROMO SUCCINAMIDE AND AMARNTH DYE COUPLE
ABSTRACT Simple, specific, accurate and precise UV–visible spectrophotometric methods have been developed for the estimation of five drugs viz., Dobutamine hydrochloride, Duloxetine hydrochloride, Ondansetron hyd...
A REVIEW ON BIOANALYTICAL TECHNIQUES
ABSTRACT Quantitative determination of active pharmaceutical ingredient and its degradative products in biological fluids is a challenging task for an analyst due to difficulties and uniqueness of the sample. A s...
DEVELOPMENT AND VALIDATION OF UVSPECTROPOTOMETRIC METHODS FOR QUANTITATIVE ESTIMATION OF TENOFOVIR DISPROXIL FUMARATE IN BULK & PHARMACEUTICAL DOSAGE FORM
ABSTRACT Zero-order and Area Under Curve [AUC] UV-Spectrophotometric methods have been developed and validated for the estimation of Tenofovir disproxil fumarate in bulk and its tablet formulations. The solutions...