IN VITRO PROTECTION OF HEPATOCYTES BY ALOCASIA MACRORRHIZA LEAF JUICE AGAINST CCL4 AND TYLENOL MEDIATED HEPATIC INJURY
Journal Title: International Journal of Pharmaceutical Applications - Year 2011, Vol 2, Issue 2
Abstract
The present study was carried out to investigate the antioxidative and hepatoprotective efficacy associated with Alocasia macrorrhiza leaf juice in the quest of finding new potent antioxidant and hepatoprotective molecules from nature using in vitro technology. To test the antioxidant and hepatoprotective efficacy in vitro liver slice model was used where in the hepatic damage was induced by the hepatotoxins CCl4 and Tylenol. The free radicals generated by CCl4 and Tylenol cause oxidative stress and result in injury to the hepatocytes. The extent of damage caused by these free radicals as well as evaluation of hepatoprotective and antioxidative efficacy associated with the Alocasia macrorrhiza leaf juice was measured in terms of leakage of marker enzymes of liver function viz AST, ALT and ALP in the surrounding incubation medium from the liver slices. Additionally the TBARS and Glutathione contents were measured in the liver slices. In presence of CCL4 as well as Tylenol there was increase in the levels of marker enzymes and TBARS coupled with depletion of total glutathione contents (GSH). However the leaf juice of Alocacisa macrorrhiza remarkably decreased the leakage of AST, ALT and ALP in the medium. The results obtained from TBARS and Glutathione assays were also supportive to conclude that the Alocasia macrorrhiza leaf juice as a whole possesses hepatoprotective and antioxidative efficacy when tested in vitro using rat liver slice model.
Authors and Affiliations
Bhagyashree R Patil , Sanjeevani H Bamane , Ujwala R Khadsare
Keywords
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