Production of a thermostable extracellular amylase from thermophilic Bacillus species
Journal Title: Journal of Pharmaceutical Sciences and Research - Year 2010, Vol 2, Issue 3
Abstract
Isolation and identification of thermophilic Bacillus sp was carried out from a soil sample. The cells were cultivated in a medium containing soluble starch as sole carbon source. The addition of calcium (10 mM) or peptone (1%) and yeast extract (0.5%) to the medium shortened the lag period and improved the growth and amylase synthesis. The optimum temperature for amylase production was detected as 35°C. Amylase production occurred at pH 5.0-9.0 with a maximum at pH 7.0. The optimal pH and temperature values for extracellular activity were 7.5 and 50°C respectively. Effects of different salts were noted and it was found that CaCl2 with concentration of 0.2g/l played an important role for optimum production and stability of alpha amylase in the fermentation medium. Starch with a concentration of 20 g/l was a good source for the enzyme synthesis. The levels of the amylase production detected in culture supernatants varied greatly with the type of carbon source used. Lactose, soluble starch and glucose stimulated amylase production. Effect of different nitrogen sources revealed that peptone increase the enzyme yield. The concentration of yeast extract was an important factor for the synthesis of amylase by the isolate. The activity of the enzyme increased between 2 and 4 g/l yeast extract concentrations with an optimum of 4 g/l. The optimal concentration of peptone for the production of amylase was detected as 10g/l.
Authors and Affiliations
Suman. S , K. Ramesh
Keywords
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