UV-SPECTROPHOTOMETRIC AND RP-HPLC METHODS FOR THE ESTIMATION OF TROXERUTIN IN BULK AND TABLET FORMULATION
Journal Title: Int J of Preclin & Pharm Res - Year 2013, Vol 3, Issue 1
Abstract
ABSTRACT Troxerutin is used as an anti-coagulant drug for the treatment of Hemiplegia, Aphasia, Cardiac stem arteriosclerosis, etc. The aim of the present study is to develop a new analytical method for the estimation of Troxerutin in bulk and in tablet dosage form. Spectroscopic method (method-1) and RP-HPLC (method-2) method have been developed for the quantification of Troxerutin in bulk and in the formulation. These methods are simple, cost effective, accurate and precise. In method-1, Troxerutin showed maximum absorbance at 348 nm in 0.1M acetic acid which is selected as solvent for our analysis based on its stability. Beer’s law obeyed in the concentration range of 5-40 mcg / ml. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 0.2628 and 0.7966 mcg/ml respectively. In RP-HPLC (method-2), the mobile phase selected is 20 mM Phosphate buffer (pH-8): Acetonitrile: Methanol in the ratio 60:25:15%v/v. The flow rate was 1 ml/min. The linearity range was found to be 5-25 mcg /ml. The formulation OXERUTE was selected for analysis and the amount present was found to be 101.69 % and 100.98% for method 1 and 2 respectively. Both the methods are validated as per ICH guidelines. The methods were found to be simple, accurate, Precise and rapid. Keywords: Troxerutin, Hemiplegia, Aphasia, Arteriosclerosis, RP-HPLC.
Authors and Affiliations
P. V. Subash Chandra Boss *, T. Vetrichelvan , M. Jyostna , K. Pragadeesh , G. Swathy , M. Shankar
Keywords
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