Partial Characterization of Bacteriocin Produced by Halotolerant Pediococcus acidilactici Strain QC38 Isolated from Traditional Cotija Cheese
Journal Title: Polish Journal of Microbiology - Year 2016, Vol 65, Issue 3
Abstract
During a screening of lactic acid bacteria producing bacteriocin from Cotija cheese, the strain QC38 was isolated. Based on the 16S rRNA gene nucleotide sequencing (516 pb accession no KJ210322) and phylogenetic analysis, the isolate was identified as Pediococcus acidilactici. Neutralized cell-free supernatant was tested for antimicrobial activity against 17 Gram-negative and Gram-positive pathogens. Growth inhibition was achieved against Listeria monocytogenes (supplier or indication or source), Staphylococcus aureus, Vibrio vulnificus, Vibrio cholerae O1 Ogawa, Vibrio cholerae NO 01 and Salmonella enterica subsp. Enterica serovar Typhimurium. Bacteriocin-like substance, after heating at 121°C for 15 min it remained stable and its antimicrobial activity was observed at pH ranging from 1.0 to 10.0 but inactivated by α-chymotrypsin and proteinase K. Strain QC38 was able to grow in 1–9% NaCl concentration. The plate overlay assay showed an approximate size of bacteriocin-like substance between 3.4 and 6.5 kDa. P. acidilactici QC38 harboured a plasmid that contains a gene for a pediocin (PA-1).
Authors and Affiliations
Aurea I. Morales-Estrada, Ahide Lopez-Merino, Nestor Gutierrez-Mendez, Enrico A. Ruiz, Ararceli Contreras-Rodriguez
Keywords
Related Articles
- EP ID EP81123
- DOI 10.5604/17331331.1215607
- Views 61
- Downloads 0
<strong>Bioprotective Potential of Bacteriocinogenic <em>Enterococcus gallinarum</em> Strains Isolated from Some Nigerian Fermented Foods, and of Their Bacteriocins</strong>
[i]Enterococcus gallinarum [/i]strains isolated from some Nigerian fermented foods were found to produce bacteriocins. The bacteriocins had a broad spectrum of activity against both Gram positive and negative bacteria. T...
???
Molecular methods for identification of monophasic Salmonella Typhimurium strains
Two molecular biology methods were used to differentiate [i]Salmonella enterica[/i] 1,4,[5],12:i:- strains: “[i]Salmonella[/i] Check&amp;amp;Trace microarray” (CT) and multiplex PCR (mPCR). For 92 strains in CT...
<strong>Antiquorum from Freshwater Zoosporic Isolated Fungi: Maximization of Some Ameliorative Factors</strong>
Many bacteria detect their critical cell numbers through a mechanism known as Quorum sensing (QS). Acyl homoserine lactone (AHL) mediated QS systems have been shown to operate in important human pathogens. The aim of thi...
Comparative Seroprevalence of Hepatitis A And E Viruses in Blood Donors from Wielkopolska Region, West-Central Poland
The objective of the present study was to investigate the seroprevalence of HAV and HEV in Polish blood donors (BDs). One hundred and ten randomly selected healthy BDs, living in Wielkopolska Region were tested for anti-...