Partial sequence of the genomic DNA from a Philippine isolate of the Penaeus monodon-type baculovirus (MBV) and comparison with other geographical isolate
Journal Title: Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society - Year 2011, Vol 4, Issue 3
Abstract
Partial genomic DNA sequences of a Philippine isolate of the Penaeus monodon-type baculovirus (MBV) was identified. The two partial sequences of the MBV genomic DNA from the Philippines showed 100% homology at the nucleotide level, indicating the occurrence of a single strain. It shared at least 87% homology of their nucleotides with the partial sequences of MBV isolates from Taiwan and India. Moreover, the sequences of the genomic DNA from the Philippine isolates clustered together and were distinct from either the Taiwan and Indian isolates. A set of primers that will yield an amplicon size of 193-bp, was designed from the least conserved region of the partial sequence for the specific detection of the Philippine isolates of this virus.
Authors and Affiliations
Christopher Caipang, May Sibonga, Jane Geduspan
Keywords
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