PRELIMINARY CHARACTERIZATION, ANTIOXIDANT ACTIVITIES AND ULCER CURATIVE EFFECT OF OPUNTIA FICUS INDICA F. INERMIS ROOTS POLYSACCHARIDES IN RATS
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2013, Vol 2, Issue 4
Abstract
The present study was undertaken to investigate the in vitro antioxidant activity and the in vivo curative effect of Opuntia ficus indica f. inermis polysaccharides (OFIP) against ethanol- induced ulcer in rats. According to gas chromatography-mass spectrometry and Fourier transform infrared spectroscopy studies OFIP was an heteropolysaccharide composed of rhamnose, arabinose, fucose, mannose, glucose and galactose at the molar ratio of 4.91; 4.94; 3.87; 32.51; 7.8; 7.1. In vitro tested for their potential antioxidant activity OFIP exhibited high radical scavenging activity against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) when compared with butylated hydroxyanisole (BHA) activity. Whereas OFIP hydroxyl radical scavenging activity, reducing power and the lipid peroxidation inhibitory effect appeared low but not weak when compared with the BHA and vitamin C activities. In vivo the treatment with OFIP at oral doses 100, 200, and 400 mg/kg b.w., was found to provide a dose-dependent protection against ethanol-induced gastric ulcer by reducing the gastric juice output, enhancing the healing rate and increasing the mucus production. The antiulcerogenic activity of OMFE might be due to a possible synergistic antioxidant, anti-excretory and healing mechanisms.
Authors and Affiliations
Hichem Alimi*| Research unit of Macromolecular Biochemistry and Genetic, Faculty of Sciences of Gafsa, University of Gafsa, 2112 Gafsa, Tunisia.-Laboratory of Integrated Physiology, Faculty of Science of Bizerte, University of Carthage, 7021 Jarzouna, Bizerte, Tunisia., Zouhour Bouoni| Laboratory of Animal Ecophysiology, Faculty of Science of Sfax, University of Sfax, 3018 Sfax, Tunisia., Anwer Feriani| Laboratory of Animal Ecophysiology, Faculty of Science of Sfax, University of Sfax, 3018 Sfax, Tunisia., Najla Hfaeidh| Laboratory of Animal Ecophysiology, Faculty of Science of Sfax, University of Sfax, 3018 Sfax, Tunisia., Mohsen Sakly| Laboratory of Integrated Physiology, Faculty of Science of Bizerte, University of Carthage, 7021 Jarzouna, Bizerte, Tunisia., Khémais Ben Rhouma| Laboratory of Integrated Physiology, Faculty of Science of Bizerte, University of Carthage, 7021 Jarzouna, Bizerte, Tunisia.
Keywords
Related Articles
- EP ID EP1853
- DOI -
- Views 358
- Downloads 24
Demonstration the Expression of α-Methyl Acyl-Co EnzymeA Racemase, P63 and high- molecular-weight cytokeratin in Prostate biopsies in Khartoum State Hospitals
Prostate cancer is the major health problem throughout the world. PCa remains the most common malignancy affecting men and the second leading cause of cancer-related death of men. The immunohistochemistry plays a very im...
RELATION OF ANTIOXIDANT STATUS AND LIPOPROTEIN (a) LEVEL IN MYOCARDIAL INFARCTION PATIENTS : A CLINICAL STUDY TO EVALUATE THE ANTIOXIDANT/ LIPOPROTEIN (a) RATIO AS A NOVEL PARAMETER TO INDICATE CARDIOVASCULAR ACCIDENT
Lipoprotein (a), a prominent marker of cardiovascular accident, was first described by Berg in 1963(3). Lipoprotein (a) [Lp(a)] has been considered as one of the distinguished signature in myocardial infarction and for m...
A short Review on Stomach Specific Floating in-situ gel
Oral drug delivery is a one of the simplest routes of delivery of drugs for systemic and in certain cases local effect. Liquid oral dosage forms are convenient to administer as compared to solid dosage forms but cannot a...
ANTIDIABETIC ACTIVITY OF 2-AMINO-(5-FLUORO-2-OXOINDOLIN-3-YLIDENE) BENZOXAZOLE-5-CARBOHYDRAZIDE IN RATS
The present study was designed to evaluate the antidiabetic activity of new indole derivatives i.e 2-Amino-(5-fluoro-2-oxoindolin-3-ylidene) benzoxazole-5-carbohydrazide against alloxan induced diabetic rats. Alloxan (12...
Analysis of Biochemical Composition of Honey and its Anti-Oxidant, Phytochemical and Anti-Bacterial Properties
Honey mainly consists of sugars and water. Apart from sugars, honey also contains several vitamins especially B complex and vitamin C, together with a lot of minerals such as calcium, copper, iron, magnesium, manganese,...