Preliminary study on resynthesis of winter oilseed rape (Brassica napus L.)
Journal Title: Rośliny Oleiste - Oilseed Crops - Year 2010, Vol 31, Issue 2
Abstract
One of the most widely used methods for resynthesis of rapeseed is crossing the selected subspecies of Brassica rapa L. and Brassica oleracea L. and obtaining plants through in vitro culture of isolated embryos in the early stage of their development. In this study resynthesized rapeseed (RS) was obtained as a result of crosses between Brassica rapa ssp. chinensis var. chinensis (Chinese cabbage – pak choy), and Brassica oleracea ssp. acephala var. sabellica (curly kale). Morphological and nuclear DNA cytometric analyses of obtained plants has confirmed their hybrid phenotype and genotype. Molecular analysis carried out using 20 RAPD primers showed that resynthesized plants are distinct from Brassica napus DH lines and from cultivars of winter oilseed rape which are bred and cultivated nowadays.
Authors and Affiliations
Katarzyna Sosnowska, Laurencja Szała, Anna Olejnik, Teresa Cegielska-Taras
Keywords
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