Propolis Action in Controlling Activated T Cell Producing TNF-a and IFN-g in Diabetic Mice
Journal Title: Turkish Journal of Immunology - Year 2017, Vol 5, Issue 2
Abstract
Objective: This study was conducted to determine the suppressor effect of propolis in controlling activated T cells, also T cells expressing TNF-α and IFN-γ. Those molecules have an important role to generate acute inflammation in diabetes mellitus (DM) condition. Material and Methods: This study was done by in vivo experiment on streptozotocin-induced diabetic mice. Mice were classified into DM group (diabetic mice model without propolis ethanolic extract); propolis treatment group doses of 50, 100, and 200 mg/kg body weight; normal mice group. Effect of each treatment was observed after 14 days by flow cytometry analysis. The absolute numbers of CD4+CD62L-, CD4+TNF-α+, and CD4+IFN-γ+ were assessed from splenic cells. Results: The data showed that administration of propolis ethanolic extract for 14 days significantly decreased the number of activated T cell. Propolis also decreased the expression of TNF-α and IFN-γ by T cells. Administration of all doses of propolis caused decreased blood glucose level compared to baseline levels. Propolis administration at a dose of 100 and 200 mg/kg could suppress the expression of the pro-inflammatory cytokines. Conclusion: Propolis decreased the number of activated T cells expressing TNF-α and IFN-γ. It also inhibited naive T cells to activate and prevented hyperglycemia. Keywords: Anti-inflammatory, bee’s natural product, hyperglycemic, immunomodulator, pro-inflammatory cytokine
Authors and Affiliations
Febby Nurdiya Ningsih, Muhaimin Rifa’i
Keywords
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- EP ID EP449859
- DOI 10.25002/tji.2017.575
- Views 88
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