Propolis Action in Controlling Activated T Cell Producing TNF-a and IFN-g in Diabetic Mice
Journal Title: Turkish Journal of Immunology - Year 2017, Vol 5, Issue 2
Abstract
Objective: This study was conducted to determine the suppressor effect of propolis in controlling activated T cells, also T cells expressing TNF-α and IFN-γ. Those molecules have an important role to generate acute inflammation in diabetes mellitus (DM) condition. Material and Methods: This study was done by in vivo experiment on streptozotocin-induced diabetic mice. Mice were classified into DM group (diabetic mice model without propolis ethanolic extract); propolis treatment group doses of 50, 100, and 200 mg/kg body weight; normal mice group. Effect of each treatment was observed after 14 days by flow cytometry analysis. The absolute numbers of CD4+CD62L-, CD4+TNF-α+, and CD4+IFN-γ+ were assessed from splenic cells. Results: The data showed that administration of propolis ethanolic extract for 14 days significantly decreased the number of activated T cell. Propolis also decreased the expression of TNF-α and IFN-γ by T cells. Administration of all doses of propolis caused decreased blood glucose level compared to baseline levels. Propolis administration at a dose of 100 and 200 mg/kg could suppress the expression of the pro-inflammatory cytokines. Conclusion: Propolis decreased the number of activated T cells expressing TNF-α and IFN-γ. It also inhibited naive T cells to activate and prevented hyperglycemia. Keywords: Anti-inflammatory, bee’s natural product, hyperglycemic, immunomodulator, pro-inflammatory cytokine
Authors and Affiliations
Febby Nurdiya Ningsih, Muhaimin Rifa’i
Keywords
Related Articles
- EP ID EP449859
- DOI 10.25002/tji.2017.575
- Views 91
- Downloads 0
Identification of Hemagglutinin Protein from Streptococcus pneumoniae Pili as a Vaccine Candidate by Proteomic Analysis
Introduction: Streptococcus pneumoniae is a Gram-positive, bacterial human pathogen that causes a large number of respiratory tract infections, such as pneumonia and sinusitis, as well as invasive diseases such as septic...
Effect of Monoclonal Antibody to Human Zona Pellucida 3 in Luteineizing Hormone Receptor Expression, Estradiol Levels and de Graaf Follicles Quantity in Mice
Objective: This research aimed to evaluate effect of monoclonal antibody to human Zona Pellucida 3 (Mab-hZP3) on luteineizing hormone (LH) receptor expression, estradiol levels and de Graaf follicles quantity of mus musc...
Levels of IL-12, IL-17, and LL-37 in Acne Vulgaris
Introduction: Acne vulgaris (AV) is a chronic inflammatory disease of the pilosebaceous unit with a multifactorial pathogenesis, which includes colonization of Propionibacterium acnes (P. acnes). Increased P. acnes colon...
Kronik Granülomatöz Hastalarında ve Bu Hastalığı Genetik Olarak Taşıyan Bireylerde Lenfosit Fonksiyonları
Introduction: Chronic granulomatous disease (CGD) is classically known as phagocytic system disease. However, in recent years, the observation of the development of autoimmunity, colitis-like findings during follow-up ha...
Metoklopramide Bağlı Olarak Gelişen Kounis Sendrom: Olgu Sunumu
A Patient Diagnosed with Kounis Syndrome due to the Metoclopramide: A Case Report In this case report, we mentioned that; a 23 years old and 14 week pregnant woman with no history of anaphylactic or allergic reaction an...