Purification of Quercetin by HPLC from green tea leaves and its application in cancer therapy
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2016, Vol 4, Issue 2
Abstract
Quercetin is the subject of intense research on the basis of its antioxidant, anti-inflammatory and anti-cancer activities. Antioxidant potential of Green tea (Camellia sinensis) leaves was checked by various antioxidant methods. The estimation of Flavonoid content with the help of Quercetin Standard curve and the total amount of polyphenolic contents in all extracts of Tea leaves was determined with Aluminum chloride colorimetric method for flavonoids determination. Total Phenolic Content assay was performed on standard Gallic acid and various extracts for calculating the amount of Phenolic content present. Nitric Oxide Scavenging Activity Assay and DPPH Scavenging Assay were performed for L-Ascorbic Acid as Standard along with phytochemical screening. HPLC method was employed for quantitative examination of flavonoid quercetin from various plants and the time of Retention was calculated. Antitumor properties of the extract of Green tea was determined by using Cytotoxicity assay.
Authors and Affiliations
Shipra Kalra, Kanav Midha, Sarbjit Kaur
Keywords
Related Articles
- EP ID EP33368
- DOI -
- Views 241
- Downloads 0
A review on antidepressant activity of Agomelatine
Agomelatine, a melatonergic antidepressant with a rapid onset of action, is one of the most recent drugs in the antidepressant category. Agomelatine’s antidepressant actions are attributed to its sleeppromoting and chro...
Design and characterization of olanzapine transfersomes for percutaneous administration
The aim of this research activity is to formulate Soyalecithin based transfersomal nanoparticles for percutaneous administration of Olanzapine for better management against psychotic diseases. Olanzapine transfersomes...
Validated UV spectrophotometric method for quantitative analysis of tramadol in bulk and pharmaceutical dosage form
A precise, accurate, economical and simple UV spectrophotometric method has been developed for the determination of Tramadol hydrochloride in bulk and pharmaceutical dosage form. The Tramadol hydrochloride shows maximu...
Synthesis of novel Tetrazole derivatives and their biological evaluation
Tetrazole have very important biological activity. We have synthesized the 10 novel tetrazole derivatives characterized by 1H NMR 13C NMR HRMS and evaluated antibacterial and antifungal activity. Among the compounds co...
Phytochemical profile of three selected plants of Semecarpus anacardium, Butea monosperma and Andrographis paniculata
A Phytochemical profile of three selected plant species Semecarpus anacardium, Butea monosperma and Andrographis paniculata were carried out. Crude dry powder analysis, ash value, solubility, extractive value, fluoresc...