Purification of Quercetin by HPLC from green tea leaves and its application in cancer therapy
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2016, Vol 4, Issue 2
Abstract
Quercetin is the subject of intense research on the basis of its antioxidant, anti-inflammatory and anti-cancer activities. Antioxidant potential of Green tea (Camellia sinensis) leaves was checked by various antioxidant methods. The estimation of Flavonoid content with the help of Quercetin Standard curve and the total amount of polyphenolic contents in all extracts of Tea leaves was determined with Aluminum chloride colorimetric method for flavonoids determination. Total Phenolic Content assay was performed on standard Gallic acid and various extracts for calculating the amount of Phenolic content present. Nitric Oxide Scavenging Activity Assay and DPPH Scavenging Assay were performed for L-Ascorbic Acid as Standard along with phytochemical screening. HPLC method was employed for quantitative examination of flavonoid quercetin from various plants and the time of Retention was calculated. Antitumor properties of the extract of Green tea was determined by using Cytotoxicity assay.
Authors and Affiliations
Shipra Kalra, Kanav Midha, Sarbjit Kaur
Keywords
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