SIMULTANEOUS SEPARATION OF SIX FLUOROQUINOLONES USING AN ISOCRATIC HPLC SYSTEM WITH UV DETECTION: APPLICATION TO ANALYSIS OF LEVOFLOXACIN IN PHARMACEUTICAL FORMULATIONS
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 2
Abstract
A simple, precise, specific, highly sensitive, an accurate and reproducible isocratic reversed phase HPLC method was developed for the separation of six fluoroquinolones and validated for determination of Levofloxacin in pure and in pharmaceutical formulations. One of the prominent aims for the method development is to achieve constant, reproducible separation. The selection of good method is essential if this goal is to be attained. RP-HPLC method was developed by using WELCHROM C18 Column (4.6 X 250mm, 5µm), SHIMADZU LC-20AT prominence liquid chromatograph. The mobile phase consisting of phosphate buffer (pH-3.1) and acetonitrile in the proportion of 70:30 v/v under isocratic elution at a flow rate of 1mL/min was employed. The responses were measured at 293 nm using SHIMADZU SPD-20A prominence UV-Vis detector. The retention time of Levofloxacin was found to be 3.607 min. The developed method was according to ICH guidelines. The average recoveries ranged from 99.518 - 100.20%, with %RSD less than 2%. The concentration of calibration solutions was in the range of 2-10 μg/mL and LOD and LOQ were 0.1234 and 0.3740 μg/mL respectively. The method was successfully applied to analysis of Levofloxacin in pharmaceutical formulation. It can also be extended for the determination of other five fluoroquinolones or their combinations.
Authors and Affiliations
Ravisankar Panchumarthy, Devala Rao Garikapati, Krishna Chaitanya Manukonda, Sandhya Rani Nagabhairava
Keywords
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