Screening, Isolation and Characterization of Amylase Producing Bacteria and optimization for Production of Amylase
Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2018, Vol 4, Issue 2
Abstract
Amylase is (E.C.3.2.1.1-1,4-alpha D-glucanohydrolase) an extracellular enzyme, which is involved in the starch processing industries where it breaks starch into simple sugar constituents.Amylase has extensive application in starch processing, brewing and sugar production, in textile industries and in detergent manufacturing processes. Interestingly, the first enzyme produced industrially was an amylase.In the present study, amylase producing bacteria were isolated from rice field, sugarcane field and sugarcane dump area and characterized for their morphological and biochemical properties. Then amylase activity of isolated bacterial cultures were determined and it was concluded that 3 (NN1, NN2, NN5)out of 6 bacterial colonies(NN1, NN2, NN3, NN4, NN5, NN6) were potent and their enzyme activity was more than other colonies. The potent colonies were also optimized for enzyme activity under certain conditions like different carbon sources, nitrogen sources, pH, incubation time and chlorides.Agro-industrial wastes were used as substrate for amylase production by Solid-State FermentationSSF) and we have found that wheat bran was the suitable substrate for amylase production.
Authors and Affiliations
Alka Ekka, Neha Namdeo
Keywords
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- EP ID EP415067
- DOI 10.9790/264X-0402015056.
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