Simultaneous determination and estimation of Amlodipine andPerindopril in raw and tablet formulation by stabilityindicating RP-HPLC method
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 5
Abstract
A sensitive, feasible RP-HPLC method has developed and validated for the analysis of Amlodipine with Perindopril argininein raw and tablet formulation.Successful separation of drugs products is developed on a C(18) column reversed-phase using and using mobile phase composition of Methanol:Phosphate buffer(27:73 v/v). The flow rate was adjusted to 1.1 mL/minute and the absorption maxima were observed at 270 nm utilizing Shimadzu SPD-20A Prominence UV-Vis detector.Good linearity was obtained in the range of 2-10 μg/ ml, 3-15 μg/ml, for Amlodipine,Perindopril arginine respectively. TheHPLC, tabletformulation assay shows percentage purity ranging from 99.16 to 100.18% for Amlodipine, 99.58 to 100.23% for Perindopril arginine. The mean percentage purity is 100.01% and 100.08% for Amlodipine andPerindopril arginine respectively. The chromatographic retention time ofAmlodipine andPerindopril arginine was found to be 4.2 and 7.3 minutes respectively. The tailing factor was 0.769 and 0.780 for Amlodipine and Perindopril arginine respectively. The developed method validated according to the ICH guidelines. The method was found to be applicable for determination and validation of Amlodipine andPerindopril arginine in combined tablet form.
Authors and Affiliations
unasekar Manoharan*
Keywords
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- EP ID EP386367
- DOI 10.9790/3008-1105032429
- Views 56
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