Simultaneous determination and estimation of Amlodipine andPerindopril in raw and tablet formulation by stabilityindicating RP-HPLC method
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 5
Abstract
A sensitive, feasible RP-HPLC method has developed and validated for the analysis of Amlodipine with Perindopril argininein raw and tablet formulation.Successful separation of drugs products is developed on a C(18) column reversed-phase using and using mobile phase composition of Methanol:Phosphate buffer(27:73 v/v). The flow rate was adjusted to 1.1 mL/minute and the absorption maxima were observed at 270 nm utilizing Shimadzu SPD-20A Prominence UV-Vis detector.Good linearity was obtained in the range of 2-10 μg/ ml, 3-15 μg/ml, for Amlodipine,Perindopril arginine respectively. TheHPLC, tabletformulation assay shows percentage purity ranging from 99.16 to 100.18% for Amlodipine, 99.58 to 100.23% for Perindopril arginine. The mean percentage purity is 100.01% and 100.08% for Amlodipine andPerindopril arginine respectively. The chromatographic retention time ofAmlodipine andPerindopril arginine was found to be 4.2 and 7.3 minutes respectively. The tailing factor was 0.769 and 0.780 for Amlodipine and Perindopril arginine respectively. The developed method validated according to the ICH guidelines. The method was found to be applicable for determination and validation of Amlodipine andPerindopril arginine in combined tablet form.
Authors and Affiliations
unasekar Manoharan*
Keywords
Related Articles
- EP ID EP386367
- DOI 10.9790/3008-1105032429
- Views 71
- Downloads 0
Entomotoxicity of Six Indegenous Plants Extracts in Controlling Callosobrochus Maculatus (Fabricius) (Coleoptera: Chrysomelidae) Infestation in Stored Vigna Unguiculata L.Walp
Biological investigations were conducted to assess the insecticidal activities of n-hexane leaf extract of Solanum tuberosum, Annona muricata, Cymbopogon citrates, Vernonia amygddalina, Caesalpinia pulcherima and Lantana...
Congenital Chloride Diarrhoea
Congenital chloride losing diarrhea (CLD) is a medical emergency case in pediatrics. It is a rare genetic disorder caused due to mutation of chromosome 7.It is commonly seen in Saudi Arabic countries ,It is seen in early...
Biosynthesis of Silver Nanoparticles by Food-Origin E. coli and Candida Species and Testing Its Antimicrobial Activity against Pathogenic Bacteria and Fungi
Due to the growing demand to improve an environmentally friendly, nonhazardous and cost-effective technology as a biocide to control the drug-resistant microorganism, thus in the present study; food-origin Escherichia co...
Development and Evaluation of Pelletized Delivery System for Effective Treatment of Type Ii Diabetes by Using Extrusion and Spheronization Technique
Diabetes mellitus is a group of metabolic disorders of carbohydrate metabolism in which glucose is underutilized, producing hyperglycemia resulting from a defect in insulin secretion, insulin action, or both. Type 2 diab...
Evaluating the Protective Impact of Ginger Extract Against Ciprofloxacin-Induced Hepatotoxicity in Male Albino rats
In the present study, potential protective impact of ginger extract against ciprofloxacin (CPFX)- induced hepatotoxicity in male rats was investigated. Forty-two adult male albino rats were divided into seven groups: con...