SPECIES IDENTIFICATION, ANTIFUNGAL SUSCEPTIBILITY TESTING AND GENETIC VARIABILITY AMONG CANDIDA SPECIES ISOLATED FROM CLINICAL SAMPLES
Journal Title: Journal of Drug Discovery and Therapeutics - Year 2013, Vol 1, Issue 3
Abstract
Candida albicans is an opportunistic pathogen that usually lives as commensal in the healthy human host. The most common infecting species is C. albicans where as other non albicans species are C. glabrata, C. parapsilosis, C. tropicalis and C. krusei that also causes various clinical diseases. Candidemia is the fourth leading cause of bloodstream infections and carries a 35–55% mortality. There is increase in the incidence of candidiasis cases has also been reported in immunocompromised individuals associated with non albicans species, such as C. glabrata, C. krusei, C. tropicalis and C. parapsilosis. The main risk factors are Invasive candidiasis, urinary candidiasis, gastrointestinal candidiasis, respiratory candidiasis and vulvo-vaginal candidiasis. MATERIAL AND METHOD: A total of 30 samples were collected from zonal hospital Solan (H.P) India and college students of SILB Solan. Candida species were isolated from 24 samples, 6 samples were found negative. Samples were further identified on the basis of phenotypic characteristics such as Germ tube test, pseudohyphae production, Chlamydospore production, morphology on CHROM agar, sugar fermentation test and sugar assimilation test. Antifungal susceptibility testing of the Candida isolates were done against amphotericin B and voriconazole. Fifty percent of the isolates were found resistant to voriconaazole, whereas, 60% were resistant to amphotericin B. However 13% isolates were found semi dose dependent to amphotericin B. Genetic variability of highly resistant Candida species i.e. Candida tropicalis (H1 and H15), Candida albicans (H2 and H8) and Candida glabrata (H6) was done by RFLP using Eco RI enzyme. In case of Candida tropicalis isolates (H1 and H15) band patterns were similar, but in case of Candida albicans (H2 and H8) the band patterns generated were dissimilar. This showed that Candida albicans from Solan geographical region showed genetic variability.
Authors and Affiliations
Surjeet singh*| Department of Microbiology, Shoolini Institute of Life Sciences and Business Management, Solan (H.P) 173212, Amit kumar| Faculty of Biotechnology, Shoolini University of Biotechnology and Management Sciences, Solan (H.P) 173212., Ajay Kumar| Department of Microbiology, Shoolini Institute of Life Sciences and Business Management, Solan (H.P) 173212
Keywords
Related Articles
- EP ID EP1349
- DOI -
- Views 488
- Downloads 38
ARTEROLANE MALEATE AND PIPERAQUINE PHOSPHATE: A NEW OPTION IN THE TREATMENT OF PLASMODIUM FALCIPARUM MALARIA
Malaria is holoendemic in India, claiming thousands of deaths annually. Recent data suggests that the burden of malaria, the most significant parasitic disease in the world, is increasing. India’s expansive geography and...
Formulation and evolution of matrix tablets- a review
Formulations that are able to control the release of drug have become an integral part of the pharmaceutical industry. Many of the pharmaceutical dosage form are formulated as sustained release dosage form to retard the...
A concrete and ethical reverences for noninvasive prenatal diagnosis of fetal aneuploidy present in maternal blood
Down's syndrome could be diagnosed before conception (prenatally) or after life commencement (postnatally). Throughout pregnancy, there are two sorts of tests that could be carried out to search for Down's syndrome - a s...
INTRODUCTION OF NEW ANTIMICROBIALS: CATIONIC PEPTIDES
Antimicrobial cationic peptides are of interest because they can combat multi-drug-resistant microbes. Most peptides form -helices or -sheet-like structures that can insert into and subsequently disintegrate negatively...
Study of Possible Correlation between Pancreatitis and Daily Consumption of Catha edulis in Experimental Animals
Khat (Catha edulis) is widely used throughout the world especially in Yemen and East Africa; It becomes a serious public health phenomena in Yemen that affects the health features of life. The present study evaluates the...