Spectrum of Haemoglobinopathies in Sikh population of Bangalore: A pilot study
Journal Title: IP Journal of Diagnostic Pathology and Oncology - Year 2018, Vol 3, Issue 2
Abstract
Objectives To study the burden of different Hemoglobinopathies in the Sikh community of Bangalore To study their patterns on gel electrophoresis EPH and compare the results with hematological parameters and peripheral smear To confirm the results by cation exchange High Performance Liquid Chromatography CEHPLC wherever necessaryMaterials and Methods A prospective pilot study was conducted on one hundred and fifty one cases who visited Gurudwara for a regular visit every Sunday About 23 ml intravenous blood samples were collected after obtaining informed consent from each individual Background data of each individual were recorded like age sex caste place of origin consanguinity etc Hematological indices were measured using MS4 Cell Counter Hemoglobin electrophoresis was carried out for the quantification of A2 fraction of hemoglobin by elution method The use of high performance liquid chromatography is used to separate and quantify abnormal haemoglobin Hb fractions that were inseparable on Gel electrophoresisResults Most common hemoglobinopathies observed were betaminusthalassemia trait 53 thalassemia major 06 Hb D trait 19 HbDbetaminusthalassemia 06 sickle cell trait 06 HbQ disease 06Conclusions Proper screening camps and counselling needs to be done on the population at risk about awareness and detection of heterozygous carriers to prevent the birth of homozygous Beta Thalassemia Major ChildKeywords Sikh population Hemoglobinopathies Electrophoresis High Power Liquid Chromatography
Authors and Affiliations
Amrit Kaur Kaler, Uma Bai KR, Vishwanathan V, Chandrashekhar P
Keywords
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- EP ID EP482772
- DOI 10.18231/2581-3706.2018.0028
- Views 122
- Downloads 0
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