Stability indicating method development and validation for the simultaneous estimation of Cyclosporin and Danazole by using RP HPLC in bulk and pharmaceutical dosage forms
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2017, Vol 5, Issue 4
Abstract
A simple and selective LC method is described for the determination of Cyclosporine and Danazole in tablet dosage forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixture of 55 volumes of water and 45 volumes of Methanol with detection of 295 nm. Linearity was observed in the range 40-140 µg/ml for Cyclosporine (r2=0.988) and 40-140 µg /ml for Danazole (r2 =0.992) for the amount of drugs estimated by the proposed methods was in good agreement with the label claim. The proposed methods were validated. The accuracy of the methods was assessed by recovery studies at three different levels. Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods and can be used for routine analysis of pharmaceutical dosage form.
Authors and Affiliations
Anusha Malladi, M. Aseervadamma
Keywords
Related Articles
- EP ID EP33466
- DOI -
- Views 322
- Downloads 0
Fabrication, Characterisation and Evaluation of Rivastigmine Loaded Transdermal Films for the Patients Enduring with Alzheimer’s disease
Alzheimer’s disease is the most common type of dementia which makes up 50 – 70% cases. Various cholinesterase inhibitors are used to treat conditions related to dementia. Among many drugs Rivastigmine is most promising...
Development and validation RP-HPLC method for simultaneous estimation of Sulfamethoxazole and Trimethoprim
A simple, economic, selective, precise, and accurate Reverse phase High Performance Liquid Chromatography method for analysis of Sulfamethoxazole and Trimethoprim, was developed and validated according to ICH guideline...
FORMULATION AND E VALUATION OF FAMOTIDINE FAST DISSOLVING TABLETS BY DIRECT COMPRESSION METHOD
Fast dissolving tablets were highly accepted drug delivery system. Fast dissolving tablets were dissolved/ disintegrated in the mouth within a matter of few seconds without need of water. The aim of the present study wa...
Phytochemical evaluation of Cassia alata, Techolospermum jasminoides and Caesalpinia sappan
In the present study, an attempt was made to investigate phytochemical evaluation of Cassia alata, Techolospermum jasminoides and Caesalpinia sappan. The crude drug powder extracts of the above plants were taken for th...
Phytochemical screening of ethanolic extract of Passiflora foetida( Linn) and medicinal importance
India is enriched with many medicinal plants. Passiflora foetida is one of among such medicinal plants. The secondary metabolites present in medicinal plants are used for the treatment of many diseases. The secondary m...