STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF TRAVOPOST AND TIMOLOL IN BULK AND PHARMACEUTICAL DOSAGE FORMS
Journal Title: Int J of Pharm Rev& Res - Year 2015, Vol 5, Issue 3
Abstract
A Simple, precise and stability indicating RP-HPLC method for simultaneous estimation of Travopost and Timolol in bulk and pharmaceutical dosage form has been developed and validated.The chromatographic separation was carried out on Intersil ODS (150X4.6mm, 5μ) column using acetonitrile: buffer in the ratio of 10:90 % v/v as mobile phase .The flow rate was 1ml/min and effluent was detected at 213nm.The retention time of Travopost and Timolol were found to be 2.516 and 6.198min respectively. The method was linear over the concentration range of 12.5-75 μg/ml and 10-60 μg/ml for Timolol and Travopost respectively. The method was found to be precise as indicated by the repeatability analysis showing %RSD less than 2.Percentage recoveries for Travopost and Timolol were 100.27 and 100.41% respectively .All the validation parameters were determined and found within the limits as per ICH guidelines, which indicates validity of the method. Travopost and Timolol subjected to the stress conditions of acid, base, peroxide, thermal, UV& neutral degradation. The degradation products were well resolved from the main peak and its impurities, proving stability indicating ability of the method
Authors and Affiliations
R. V. Valli Kumari , P. VenkateswarRao , A. Sunitha , K. Keerthy
Keywords
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- EP ID EP106565
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