STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF TRAVOPOST AND TIMOLOL IN BULK AND PHARMACEUTICAL DOSAGE FORMS
Journal Title: Int J of Pharm Rev& Res - Year 2015, Vol 5, Issue 3
Abstract
A Simple, precise and stability indicating RP-HPLC method for simultaneous estimation of Travopost and Timolol in bulk and pharmaceutical dosage form has been developed and validated.The chromatographic separation was carried out on Intersil ODS (150X4.6mm, 5μ) column using acetonitrile: buffer in the ratio of 10:90 % v/v as mobile phase .The flow rate was 1ml/min and effluent was detected at 213nm.The retention time of Travopost and Timolol were found to be 2.516 and 6.198min respectively. The method was linear over the concentration range of 12.5-75 μg/ml and 10-60 μg/ml for Timolol and Travopost respectively. The method was found to be precise as indicated by the repeatability analysis showing %RSD less than 2.Percentage recoveries for Travopost and Timolol were 100.27 and 100.41% respectively .All the validation parameters were determined and found within the limits as per ICH guidelines, which indicates validity of the method. Travopost and Timolol subjected to the stress conditions of acid, base, peroxide, thermal, UV& neutral degradation. The degradation products were well resolved from the main peak and its impurities, proving stability indicating ability of the method
Authors and Affiliations
R. V. Valli Kumari , P. VenkateswarRao , A. Sunitha , K. Keerthy
Keywords
Related Articles
- EP ID EP106565
- DOI -
- Views 141
- Downloads 0
PRESCRIBING PATTERN OF DRUGS IN PEDIATRIC WARDS AT A TERTIARY CARE TEACHING HOSPITAL
Pediatrics differs from adult medicine in many respects. Inappropriate prescribing is a recognized worldwide problem of the health care delivery system. Antibiotics are the widely used drugs in modern medical care. A pro...
FORMULATION AND EVALUATION OF NEVIRAPINE LOADED CHITOSAN AND EUDRAGIT NANOPARTICLES FOR ANTIRETROVIRAL THERAPY
The goal of the present investigation was to formulate and evaluate chitosan and Eudragit® nanoparticles of nevirapine for antiretroviral therapy. Nanoparticles of nevirapine were prepared using chitosan, Eudragit® S 100...
EFFECTS OF LYCOPENE COMPUND ON OXIDATIVE STRESS TO REDUCE THE RATE OF CARDIAC COMPLICATIONS IN TYPE – II DIABETES MELLITUS
The objective of the present study is to evaluate the beneficial effect of tomatoes, which are rich source of Lycopene, relatively a new carotenoid known to play an important role in human health and disease. In this stu...
ANTHELMINTIC AND ANTIMICROBIAL PROPERTIES OF LEAVES OF BRUGUIERA GYMNORRHIZA (L.) LAM
The objective of the present investigation was to determine the anthelmintic and antimicrobial activity of petroleum ether extract of the leaves of Bruguiera gymnorrhiza (L.) Lam. Anthelmintic activity of this extract wa...
REGULATORY APPROVAL OF GENERICS IN CANADA MARKET
The present thesis determines the regulations and regulatory approval process of generics in Canada. Canada is the largest individual market in North America. It is the 8th largest market in the world and in the pharmace...