Study of Extended Spectrum Beta Lactamase and Metallo Beta Lactamase Production among Gram Negative Clinical Isolates from a Tertiary Care Hospital, North-East India
Journal Title: International Journal of Medical Research Professionals - Year 2018, Vol 4, Issue 4
Abstract
Introduction: Antibiotic resistance among Gram negative bacteria is a rapidly expanding problem. In Gram-negative bacteria, extended-spectrum beta-lactamases (ESBL), and metallo beta-lactamases (MBL), have emerged as a cause of antimicrobial resistance. ESBL and MBL producing isolates are often multi-drug resistant. The multidrug resistant (MDR) isolates pose threat to therapy as well as serious concerns for infection control management. Methods: A cross-sectional study was carried out at the Microbiology Laboratory of a tertiary care teaching hospital in Assam, North East India between September 2017 and January 2018. A total of 339 Gram negative isolates were included in the study. Antimicrobial susceptibility testing of the isolates was performed by Kirby-Bauer disc diffusion technique. Phenotypic identification of ESBL producing isolates was carried out using CLSI recommended combined disc diffusion test method. Combined disc diffusion test using Imipenem alone and Imipenem with EDTA discs was done for phenotypic detection of MBL production. Results: Of the 339 Gram negative isolates, 14.75% (50/339) were ESBL producers, 17.11% (58/339) were MBL producers and 3% (10/339) were both ESBL and MBL producers. Klebsiella species was the most commonly isolated ESBL as well as MBL producer. MBL was found in significantly higher percentage of isolates from inpatients than outpatients (p<0.0001). Conclusion: Infections with strains expressing ESBL or MBL are a challenge for both microbiologists and clinicians as they are having less therapeutic options. Early detection of beta-lactamase producing isolates can help in providing an appropriate antimicrobial therapy and in avoiding the development and the dissemination of these multidrug resistant strains.
Authors and Affiliations
Binita Bhuyan, Pallabi Sargiary, Reema Nath
Keywords
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- EP ID EP534254
- DOI 10.21276/ijmrp.2018.4.4.016
- Views 80
- Downloads 0
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