Study on mechanism of the regulation of mitochondrial autophagy by Hewei Liqi Decoction on gastrointestinal motility disorders in functional dyspepsia based on PINK1 / Parkin pathway

Journal Title: Journal of Air Force Medical University - Year 2023, Vol 44, Issue 6

Abstract

Objective To observe the effects of Hewei Liqi Decoction on gastrointestinal motility and PINK1 / Parkin signaling pathway in functional dyspepsia ( FD ) rat model with liver-stomach disharmony, and to explore its regulatory mechanism on mitochondrial autophagy. Methods Forty healthy Wistar rats were randomly divided into blank control group, model control group, mosapride group, and Hewei Liqi Decoction group, with 10 rats in each group. Except for the blank control group, the other groups were made into FD rat model with liver-stomach disharmony by clipping tails. After the model was established, the Hewei Liqi Decoction group and the mosapride group were treated with medication. The general state, water intake, food intake and organic changes on the surface of gastric mucosa were observed. The gastric emptying rate and small intestinal propulsion rate were detected to evaluate the gastrointestinal motor function. HE staining was used to observe the pathological changes of gastric antrum such as erosion, ulceration, metaplasia, atrophy and atypical hyperplasia. Immunofluorescence staining was used to detect the changes of mitochondrial autophagy in gastric tissues, JC-1 flow cytometry technology to detect the changes of mitochondrial membrane potential, enzyme linked immunosorbent assay to analyze the content of mitochondrial adenine nucleoside triphosphate ( ATP) and the level of reactive oxygen species (ROS), and Western blotting to detect the expressions of mitochondrial autophagy protein PINK1, LC3, Parkin, and P62. Results Compared with the blank control group, the water intake and food intake of the model control group significantly decreased (all P < 0. 05), the gastric emptying rate and small intestinal propulsion rate slowed down (all P < 0. 05), CYT-C quickly expressed in gastric tissues, LC3B expression significantly accumulated, mitochondrial membrane potential downregulated (P < 0. 05), the proportion of mitochondrial membrane potential changes significantly up-regulated (P < 0. 05), the ability of mitochondria to generate ATP significantly down-regulated (P < 0. 05), mitochondrial ROS levels significantly increased (P < 0. 05), and the expression of mitochondrial autophagy protein PINK1, Parkin, P62 and LC3 significantly increased (all P < 0. 05). Compared with the model control group, the water intake and food intake of rats in the mosapide group and Hewei Liqi Decoction group increased significantly (all P < 0. 05), the gastric emptying rate and small intestinal propulsion rate increased significantly ( all P < 0. 05 ), the expression of CYT-C was inhibited, LC3B accumulation decreased significantly, mitochondrial membrane potential up-regulated significantly (P < 0. 05), the proportion of changes of mitochondrial membrane potential down-regulated significantly (P < 0. 05), the ability of mitochondria to generate ATP up-regulated significantly (P < 0. 05), mitochondrial ROS levels significantly down-regulated (P < 0. 05), the ROS level of Hewei Liqi Decoction group was down-regulated more significantly ( P < 0. 01 ), and the expression of mitochondrial autophagy protein PINK1, Parkin, P62 and LC3 significantly down-regulated ( all P < 0. 05). Conclusion There are gastrointestinal motility disorders in FD, and Hewei Liqi Decoction increases gastrointestinal motility and improves the symptoms of FD rats with liver-stomach disharmony by regulating mitochondrial autophagy mediated by PINK1/ Parkin signaling pathway.

Authors and Affiliations

BAI Lu

Keywords

Related Articles

Exploration of an integrated screening model of symptom-trait-eye movement for high-risk population of obsessive-compulsive disorder under the framework of item response theory

Objective To explore the symptom-trait-eye movement integration screening strategy in high-risk population of obsessive-compulsive disorder (OCD). Methods The eye tracking experiment was used to analyze the eye movement...

Analysis and reflection on survey results for oral diseases in 420 officers and soldiers of a western unit

Objective To investigate the oral health status of grass-roots troops, and combined with the investigation results, put forward the diagnosis and treatment range of oral sergeants in grass-roots troops for reference. Me...

Development and challenges of neuro-stimulus fusion to consciousness

The research of neuro-stimulus fusion to consciousness (NSFC) has gone through eight years. It does not abandon psychological measurement, one of the traditional methods of consciousness research, but gives new life to...

Alterations of NR1 expression and phosphorylation in the medial prefrontal cortex during neuropathic pain accompanied by negative emotions

Objective To study alterations of the NR1 subunit of N-methyl-D-aspartate receptors (NMDARs) in the expression and phosphorylation in medial prefrontal cortex (mPFC) brain region under the condition of neuropathic pain...

Prevention and treatment of oxidative stress injury of vitiligo based on the TCM theory of “preventive treatment of disease”

The TCM theory of “preventive treatment of disease” can play a role in the prevention and treatment of many diseases. We found that the three states in the TCM theory of “preventive treatment of disease”, namely healthy...

Download PDF file
  • EP ID EP727735
  • DOI -
  • Views 39
  • Downloads 0

How To Cite

BAI Lu (2023). Study on mechanism of the regulation of mitochondrial autophagy by Hewei Liqi Decoction on gastrointestinal motility disorders in functional dyspepsia based on PINK1 / Parkin pathway. Journal of Air Force Medical University, 44(6), -. https://europub.co.uk/articles/-A-727735