THE COMPARISON OF ANALYTICAL METHODS USED TO DETERMINE THE G-CLASS IMMUNOGLOBULINS LEVEL IN COWS SERUM AND COLOSTRUM
Journal Title: Zeszyty Naukowe Uniwersytetu Przyrodniczego we Wrocławiu, Biologia i Hodowla Zwierząt - Year 2011, Vol 583, Issue 0
Abstract
The research material were samples of colostrum and blood collected from 12 cows of the Polish Holstein-Friesian breed, black and white type. Colostrum was collected from the first full milking after the parturition. Blood samples were collected in the 4[sup]th[/sup] week after calving. The two kind of analyses was used to mark the level of G-class immunoglobulins in the blood and colostrums samples: one of them was the ELISA test (Bethyl Inc. kit), and the second one was a electrophoresis method based on polyacrylamide gel. The accuracy of the used analysis method was specified, also the regression and correlation coefficient between obtained results was set. Used methods were compared in the range of duration time and ease of indications. In the analysis of colostrums and serum done by the electrophoresis method, the IgG level was lower with the higher standard deviations value which means the higher variability of values obtained and consequently lower precision of the analysis. The correlation coefficient between the values obtained by these methods was 0.76 for serum, and 0.66 for colostrum samples. These values mean a positive linear relationship between variables. As a result, theoretically, we can compare the results obtained by ELISA test and by electrophoresis method. The methods described have a similar ease of an implementation. They can be used both in quantitative and qualitative analysis. However there are different as regards time consumption. Analysis of 44 samples of serum and colostrum by electrophoresis method took about 30 hours to make, while marking the level of immunoglobulin on a single plate in the ELISA test (maximum 40 samples) took around 20 hours.
Authors and Affiliations
Ewa Pecka, Andrzej Zachwieja
Keywords
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