The effect of hypoxic preconditioning on the sensitivity of neurons of the hippocampal СА1 area in the experimental brain ischemia
Journal Title: Український неврологічний журнал - Year 2019, Vol 0, Issue 1
Abstract
Objective — to determine the neuroprotective effects of preconditioning (PC) on the structure of hippocampal CA1 area in ischemic Mongolian gerbils. Methods and subjects. Study of PC effects was carried out on gerbil males which was divided into 4 groups: 1 — control animals, 2 — group of animals with brain ischemia, 3 — group of animals with PC without ischemia, 4 — group of animals with PC + ischemia. Before ischemia were used interval hypoxic trainings (5 times for 5 minutes at 5‑minute intervals every day for 21 days) using normobaric hypoxic gas mixtures (10 % O2 in N2). Global ischemia was modeled by occlusion of both carotid arteries. The brain tissue was taken 7 days after the occlusion. The thin sections of hippocampus were used for studying a degree of ischemic damage of pyramidal neurons and astrocytes in CA1 area with light microscopy and immunohistochemical methods. Results. The structural analysis showed the high level of delayed neuronal death in hippocampal CA1 area on the seventh day after 5‑minute occlusion in the ischemiс group. In the group of animals with PC + ischemia the structural damage of this zone was less evident. Immunohistochemical analysis revealed that the number of survived neurons was significantly higher compared with the group with ischemia. In addition, there was a tendency to reduction of astrocyte’s post‑ischemic activation in the PC + ischemia group, although the number of astrocytes was higher than in the control group. Conclusions. Analysing the effects of hypoxic PC in the hippocampal CA1 area under cerebral ischemia it was found the increasing of the guantity of survived pyramidal neurons on the seventh day after ischemia. The number of these neurons was 28.5 % higher compared with ischemia group. The increase in the number of survived neurons and the reduction of postischemic activation of astrocytes demonstrate the initiation of long‑term endogenous mechanisms of neuroprotection by preconditioning.
Authors and Affiliations
T. M. Kovalenko, I. O. Osadchenko, N. V. Chaika, G. G. Skybo
Keywords
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- EP ID EP594474
- DOI 10.30978/UNJ2019-1-75
- Views 110
- Downloads 0
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